Accelerated Wound Closure - Differently Organized Nanofibers Affect Cell Migration and Hence the Closure of Artificial Wounds in a Cell Based In Vitro Model.

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  • Additional Information
    • Source:
      Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
    • Publication Information:
      Original Publication: San Francisco, CA : Public Library of Science
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    • Abstract:
      Nanofiber meshes holds great promise in wound healing applications by mimicking the topography of extracellular matrix, hence providing guidance for crucial cells involved in the regenerative processes. Here we explored the influence of nanofiber alignment on fibroblast behavior in a novel in vitro wound model. The model included electrospun poly-ε-caprolactone scaffolds with different nanofiber orientation. Fibroblasts were cultured to confluency for 24h before custom-made inserts were removed, creating cell-free zones serving as artificial wounds. Cell migration into these wounds was evaluated at 0-, 48- and 96h. Cell morphological analysis was performed using nuclei- and cytoskeleton stainings. Cell viability was assessed using a biochemical assay. This study demonstrates a novel in vitro wound assay, for exploring of the impact of nanofibers on wound healing. Additionally we show that it's possible to affect the process of wound closure in a spatial manner using nanotopographies, resulting in faster closure on aligned fiber substrates.
      Competing Interests: All authors are co-founders of- and, to different degree affiliated with Cellevate AB (MO and AJ are now employed, FJ have no active role). However, no financial support for this work has been provided by Cellevate AB that could have influenced its outcome. The authors affiliation with Cellevate AB does neither alter our adherence to PLOS ONE policies on sharing data and materials, nor affecting any patents, products in development, or marketed products etc.
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    • Publication Date:
      Date Created: 20170107 Date Completed: 20170815 Latest Revision: 20181113
    • Publication Date:
      20240829
    • Accession Number:
      PMC5218412
    • Accession Number:
      10.1371/journal.pone.0169419
    • Accession Number:
      28060880