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Regulation of PPARγ and CIDEC expression by adenovirus 36 in adipocyte differentiation.
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- Additional Information
- Source:
Publisher: Springer Country of Publication: Netherlands NLM ID: 0364456 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-4919 (Electronic) Linking ISSN: 03008177 NLM ISO Abbreviation: Mol Cell Biochem Subsets: MEDLINE
- Publication Information:
Publication: New York : Springer
Original Publication: The Hague, Dr. W. Junk B. V. Publishers.
- Subject Terms:
- Abstract:
This study is to investigate the role of adenovirus 36 (Ad36) in regulating expression of peroxisome proliferator-activated receptor γ (PPARγ) and cell death-inducing DFFA-like effector c (CIDEC) in Ad36-induced adipocyte differentiation. Human adipose-derived mesenchymal stem cells (hAMSCs) were isolated and cultured, and then infected with Ad36. Ad36-induced adipocytes were identified using quantitative real-time PCR and Oil red O staining. The expression levels of PPARγ and CIDEC in Ad36-induced adipocytes were determined by quantitative real-time PCR and Western blot analysis. Glucose uptake and intracellular triglyceride content were also determined in these induced cells. Our results from the Oil red O staining showed that Ad36 induced the differentiation of hAMSCs into human adipocytes in vitro. Moreover, the medium glucose concentration was significantly decreased, while the intracellular triglyceride content was significantly increased, in the Ad36-induced adipocytes, compared with the control group. Furthermore, our results showed that, the mRNA and protein expression levels of PPARγ and CIDEC were significantly upregulated in Ad36-induced adipocytes, in a time-dependent manner. On the other hand, compared with the control group, the CIDEC expression was downregulated when the Ad36-induced adipocytes were treated with the PPARγ inhibitor, GW9662. Ad36 could upregulate the expression level of CIDEC through increasing PPARγ expression during the adipocyte differentiation process.
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- Contributed Indexing:
Keywords: Adenovirus 36 (Ad36); CIDEC; Human adipose-derived mesenchymal stem cell (hAMSCs); PPARγ
- Accession Number:
0 (2-chloro-5-nitrobenzanilide)
0 (Anilides)
0 (Apoptosis Regulatory Proteins)
0 (CIDEC protein, human)
0 (PPAR gamma)
0 (Proteins)
0 (Triglycerides)
IY9XDZ35W2 (Glucose)
- Publication Date:
Date Created: 20161221 Date Completed: 20170414 Latest Revision: 20191210
- Publication Date:
20221213
- Accession Number:
10.1007/s11010-016-2912-x
- Accession Number:
27995415
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