tRNA-Derived RNA Fragments Associate with Human Multisynthetase Complex (MSC) and Modulate Ribosomal Protein Translation.

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  • Additional Information
    • Source:
      Publisher: American Chemical Society Country of Publication: United States NLM ID: 101128775 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1535-3907 (Electronic) Linking ISSN: 15353893 NLM ISO Abbreviation: J Proteome Res Subsets: MEDLINE
    • Publication Information:
      Original Publication: Washington, D.C. : American Chemical Society, c2002-
    • Subject Terms:
      Protein Biosynthesis*; Ligases/*genetics ; Multienzyme Complexes/*genetics ; RNA, Transfer/*genetics ; RNA-Binding Proteins/*genetics ; Ribosomes/*genetics; Base Sequence ; Computational Biology ; Immunoprecipitation ; Isotope Labeling ; Ligases/metabolism ; Multienzyme Complexes/metabolism ; Poly A/genetics ; Poly A/metabolism ; RNA, Transfer/metabolism ; RNA, Untranslated/genetics ; RNA, Untranslated/metabolism ; RNA-Binding Proteins/metabolism ; Ribosomes/metabolism
    • Abstract:
      The functionality of small RNAs from abundant species of "housekeeping" noncoding RNAs (e.g., rRNA, tRNA, snRNA, snoRNA, etc.) remains a highly studied topic. The current state of research on short RNAs derived from transfer RNA (tRNA), called tRNA-derived fragments (tRFs), has been restricted largely to expression studies and limited functional studies. 5' tRFs are known translational inhibitors in mammalian cells, yet little is known about their functionality. Here we report on the first experimental evidence of the tRF protein interactome, identifying the mammalian multisynthetase complex as the primary interactor of the 5' tRF Gln19. We also present proteome-wide SILAC evidence that 5' tRFs increase ribosomal and poly(A)-binding protein translation.
    • Contributed Indexing:
      Keywords: SILAC; immunoprecipitation; mass spectrometry; multisynthetase complex; protein translation; small RNA; tRF; tRNA-derived fragment
    • Accession Number:
      0 (Multienzyme Complexes)
      0 (RNA, Untranslated)
      0 (RNA-Binding Proteins)
      24937-83-5 (Poly A)
      9014-25-9 (RNA, Transfer)
      EC 6.- (Ligases)
    • Publication Date:
      Date Created: 20161213 Date Completed: 20171016 Latest Revision: 20180403
    • Publication Date:
      20221213
    • Accession Number:
      10.1021/acs.jproteome.6b00267
    • Accession Number:
      27936807