Use of an experimental model to evaluate infection resistance of meshes in abdominal wall surgery.

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  • Additional Information
    • Source:
      Publisher: Academic Press Country of Publication: United States NLM ID: 0376340 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-8673 (Electronic) Linking ISSN: 00224804 NLM ISO Abbreviation: J Surg Res Subsets: MEDLINE
    • Publication Information:
      Publication: New York, NY : Academic Press
      Original Publication: Philadelphia [etc.]
    • Subject Terms:
      Abdominal Wall/*surgery ; Herniorrhaphy/*instrumentation ; Mycobacterium Infections, Nontuberculous/*prevention & control ; Staphylococcal Infections/*prevention & control ; Surgical Mesh/*microbiology ; Surgical Wound Infection/*prevention & control; Animals ; Biocompatible Materials ; Collagen ; Mycobacterium Infections, Nontuberculous/etiology ; Mycobacterium fortuitum/growth & development ; Polypropylenes ; Random Allocation ; Rats ; Rats, Wistar ; Staphylococcal Infections/etiology ; Staphylococcus aureus/growth & development ; Staphylococcus epidermidis/growth & development
    • Abstract:
      Background: Staphylococcal species are the most common organisms causing prosthetic mesh infections, however, infections due to rapidly growing mycobacteria are increasing. This study evaluates the resistance of biomaterial for abdominal wall prostheses against the development of postoperative infection in a rat model.
      Material and Methods: In 75 rats, we intramuscularly implanted three different types of prostheses: (1) low-density polypropylene monofilament mesh (PMM), (2) high-density PMM, and (3) a composite prosthesis composed of low-density PMM and a nonporous hydrophilic film. Meshes were inoculated with a suspension containing 10 8 colony-forming units of Staphylococcus aureus, Staphylococcus epidermidis, Mycobacterium fortuitum, or Mycobacterium abscessus before wound closure. Animals were sacrificed on the eighth day postoperatively for clinical evaluation, and the implants were removed for bacteriologic analyses.
      Results: Prostheses infected with S aureus showed a higher bacterial viability, worse integration, and clinical outcome compared with infection by other bacteria. Composite prostheses showed a higher number of viable colonies of both M fortuitum and Staphylococcus spp., with poorer integration in host tissue. However, when the composite prosthesis was infected with M abscessus, a lower number of viable bacteria were isolated and a better integration was observed compared with infection by other bacteria.
      Conclusions: Considering M abscessus, a smaller collagen-free contact surface shows better resistance to infection, however, depending on the type of bacteria, prostheses with a large surface, and covered with collagen shows reduced resistance to infection, worse integration, and worse clinical outcome.
      (Copyright © 2016 Elsevier Inc. All rights reserved.)
    • Contributed Indexing:
      Keywords: Abdominal infection; Biofilm; Prosthesis; Rapidly growing mycobacteria; Rat model; Surgical site infection
    • Accession Number:
      0 (Biocompatible Materials)
      0 (Polypropylenes)
      9007-34-5 (Collagen)
    • Publication Date:
      Date Created: 20161126 Date Completed: 20170705 Latest Revision: 20191210
    • Publication Date:
      20250114
    • Accession Number:
      10.1016/j.jss.2016.08.056
    • Accession Number:
      27884340