Hsa_circ_0054633 in peripheral blood can be used as a diagnostic biomarker of pre-diabetes and type 2 diabetes mellitus.

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  • Additional Information
    • Source:
      Publisher: Springer Verlag Country of Publication: Germany NLM ID: 9200299 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-5233 (Electronic) Linking ISSN: 09405429 NLM ISO Abbreviation: Acta Diabetol Subsets: MEDLINE
    • Publication Information:
      Publication: Berlin : Springer Verlag
      Original Publication: Berlin : Springer International, c1991-
    • Subject Terms:
      Diabetes Mellitus, Type 2/*blood ; Diabetes Mellitus, Type 2/*diagnosis ; Prediabetic State/*blood ; Prediabetic State/*diagnosis ; RNA/*blood; Adult ; Aged ; Aged, 80 and over ; Biomarkers/blood ; Case-Control Studies ; Cohort Studies ; Diabetes Mellitus, Type 2/genetics ; Down-Regulation ; Female ; Humans ; Male ; Microarray Analysis ; Middle Aged ; Prediabetic State/genetics ; Prognosis ; RNA, Circular ; Sensitivity and Specificity
    • Abstract:
      Aims: The purpose of the current study was to investigate the characteristic expression of circular RNAs (circRNAs) in the peripheral blood of type 2 diabetes mellitus (T2DM) patients and their potential as diagnostic biomarkers for pre-diabetes and T2DM.
      Methods: CircRNAs in the peripheral blood from six healthy individuals and six T2DM patients were collected for microarray analysis, and an independent cohort study consisting of 20 normal cases, 20 pre-diabetes patients and 20 T2DM patients was conducted to verify the five chosen circRNAs. We then tested hsa_circ_0054633 in a third cohort (control group, n = 60; pre-diabetes group, n = 63; and T2DM group, n = 64) by quantitative real-time polymerase chain reaction (Q-PCR).
      Results: In total, 489 circRNAs were discovered to be differentially expressed between the two groups, and of these, 78 were upregulated and 411 were downregulated in the T2DM group. Five circRNAs were then selected as candidate biomarkers and further verified in a second cohort. Hsa_circ_0054633 was found to have the largest area under the curve (AUC). The diagnostic capacity of hsa_circ_0054633 was tested in a third cohort. After introducing the risk factors of T2DM, the hsa_circ_0054633 AUCs for the diagnosis of pre-diabetes and T2DM slightly increased from 0.751 (95% confidence interval [0.666-0.835], P < 0.001) to 0.841 ([0.773-0.910], P < 0.001) and from 0.793 ([0.716-0.871], P < 0.001) to 0.834 ([0.762-0.905], P < 0.001), respectively.
      Conclusions: Hsa_circ_0054633 presented a certain diagnostic capability for pre-diabetes and T2DM.
    • References:
      Diabetes. 2016 Mar;65(3):803-17. (PMID: 26631737)
      Nat Biotechnol. 2014 May;32(5):453-61. (PMID: 24811520)
      Nature. 2014 Jan 16;505(7483):344-52. (PMID: 24429633)
      Diabet Med. 1998 Jul;15(7):539-53. (PMID: 9686693)
      Acta Diabetol. 2015 Oct;52(5):905-15. (PMID: 25794879)
      Clin Chim Acta. 2015 Apr 15;444:132-6. (PMID: 25689795)
      Cancer Biomark. 2016;16(1):161-9. (PMID: 26600397)
      Nature. 2013 Mar 21;495(7441):333-8. (PMID: 23446348)
      N Engl J Med. 2008 Feb 7;358(6):580-91. (PMID: 18256393)
      Elife. 2015 Jun 09;4:e07540. (PMID: 26057830)
      Front Genet. 2013 Dec 31;4:307. (PMID: 24427167)
      Lijec Vjesn. 2016 Jan-Feb;138(1-2):57-8. (PMID: 27290816)
      Nat Struct Mol Biol. 2015 Mar;22(3):256-64. (PMID: 25664725)
      Sci Rep. 2015 Jan 27;5:8057. (PMID: 25624062)
      Cell Metab. 2012 Oct 3;16(4):435-48. (PMID: 23040067)
      Transl Res. 2011 Apr;157(4):253-64. (PMID: 21420036)
      Nat Rev Genet. 2011 Nov 18;12(12):861-74. (PMID: 22094949)
      Nat Genet. 2015 Dec;47(12):1415-25. (PMID: 26551672)
      PLoS Genet. 2010 Dec 02;6(12):e1001233. (PMID: 21151960)
      Cell. 2015 Mar 12;160(6):1125-34. (PMID: 25768908)
      Nature. 2011 May 19;473(7347):337-42. (PMID: 21593866)
      Sci Rep. 2015 Jul 27;5:12453. (PMID: 26211738)
      Sci Rep. 2016 Mar 02;6:22572. (PMID: 26931159)
      RNA. 2013 Feb;19(2):141-57. (PMID: 23249747)
      Acta Diabetol. 2015 Feb;52(1):161-6. (PMID: 25026924)
      Nature. 2013 Mar 21;495(7441):384-8. (PMID: 23446346)
      Mol Cell. 2014 Oct 2;56(1):55-66. (PMID: 25242144)
      N Engl J Med. 2008 Oct 9;359(15):1577-89. (PMID: 18784090)
      Front Biosci. 2000 Jan 01;5:D1-19. (PMID: 10702380)
      Oncotarget. 2015 Mar 20;6(8):6001-13. (PMID: 25749389)
      Acta Diabetol. 2016 Dec;53(6):915-923. (PMID: 27488727)
    • Contributed Indexing:
      Keywords: Biomarker; Circular RNAs (circRNAs); Circulating circRNA; Microarray analysis; Pre-diabetes; Type 2 diabetes mellitus (T2DM)
    • Accession Number:
      0 (Biomarkers)
      0 (RNA, Circular)
      63231-63-0 (RNA)
    • Publication Date:
      Date Created: 20161124 Date Completed: 20170421 Latest Revision: 20191210
    • Publication Date:
      20221213
    • Accession Number:
      PMC5329094
    • Accession Number:
      10.1007/s00592-016-0943-0
    • Accession Number:
      27878383