Papaverine-sensitive phosphodiesterase activity is measured in bovine spermatozoa.

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  • Additional Information
    • Source:
      Publisher: Wiley-Blackwell Country of Publication: England NLM ID: 101585129 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2047-2927 (Electronic) Linking ISSN: 20472919 NLM ISO Abbreviation: Andrology Subsets: MEDLINE
    • Publication Information:
      Original Publication: Oxford : Wiley-Blackwell, 2013-
    • Subject Terms:
      Papaverine/*pharmacology ; Phosphoric Diester Hydrolases/*metabolism ; Spermatozoa/*enzymology; Acrosome Reaction/drug effects ; Animals ; Calcium/metabolism ; Cattle ; Epididymis/cytology ; Epididymis/metabolism ; Male ; Phosphorylation ; Signal Transduction/drug effects ; Signal Transduction/physiology ; Spermatozoa/drug effects
    • Abstract:
      Cyclic adenosine monophosphate (cAMP) plays a crucial role as a signaling molecule for capacitation, motility, and acrosome reaction in mammalian spermatozoa. It is well-known that cAMP degradation by phosphodiesterase (PDE) enzyme has a major impact on sperm functions. This study was undertaken to characterize cAMP-PDE activity in bovine spermatozoa. Total cAMP-PDE activity in cauda epididymal and ejaculated spermatozoa was 543.2 ± 49.5 and 1252.6 ± 86.5 fmoles/min/10 6 spermatozoa, respectively. Using different family-specific PDE inhibitors, we showed that in cauda epididymal and ejaculated spermatozoa, the major cAMP-PDE activity was papaverine-sensitive (44.5% and 57.5%, respectively, at 400 nm, papaverine is a specific inhibitor of the PDE10 family). These data are supporting the functional presence of PDE10 in bovine spermatozoa and were further confirmed by western blot to be PDE10A. Using immunocytochemistry, we showed immunoreactive signal for PDE10A present on the post-acrosomal region of the head and on the flagella of ejaculated spermatozoa. Using papaverine, we showed that it promotes tyrosine phosphorylation of sperm proteins, phosphorylation of Erk1 and Erk2, and Ca 2+ release from Ca 2+ store. These results suggest that PDE10 is functionally present in bovine spermatozoa and is affecting different molecular events involved in capacitation, most probably by cAMP local regulation.
      (© 2016 American Society of Andrology and European Academy of Andrology.)
    • Contributed Indexing:
      Keywords: cAMP; cAMP-PDE; protein phosphorylation; sperm function; spermatozoa
    • Accession Number:
      DAA13NKG2Q (Papaverine)
      EC 3.1.4.- (Phosphoric Diester Hydrolases)
      SY7Q814VUP (Calcium)
    • Publication Date:
      Date Created: 20161119 Date Completed: 20171121 Latest Revision: 20180226
    • Publication Date:
      20221213
    • Accession Number:
      10.1111/andr.12290
    • Accession Number:
      27860455