Low temperature-induced viable but not culturable state of Ralstonia eutropha and its relationship to accumulated polyhydroxybutyrate.

Publisher: Oxford University Press Country of Publication: England NLM ID: 7705721 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1574-6968 (Electronic) Linking ISSN: 03781097 NLM ISO Abbreviation: FEMS Microbiol Lett Subsets: MEDLINE

Publication: 2015- : Oxford Oxford University Press
Original Publication: Amsterdam, Published by Elsevier/North Holland on behalf of the Federation of European Microbiological Societies.

Cold Temperature*; Bacillus subtilis/*growth & development ; Cupriavidus necator/*growth & development ; Escherichia coli/*growth & development ; Hydroxybutyrates/*metabolism; Bacillus subtilis/genetics ; Colony Count, Microbial ; Cupriavidus necator/genetics ; Escherichia coli/genetics ; Hot Temperature

The culturability of Escherichia coli, Ralstonia eutropha and Bacillus subtilis after incubation in phosphate-buffered saline at either 5°C or 30°C was determined. The culturability of B. subtilis showed little dependence on temperature. The culturability of E. coli rapidly decreased at 30°C but remained almost constant at 5°C. In contrast, the culturability of R. eutropha decreased by three orders of magnitude at 5°C within 24 h but only moderately decreased (one order of magnitude) at 30°C. Remarkably, prolonged incubation of R. eutropha at 30°C resulted in a full recovery of colony forming units in contrast to only a partial recovery at 5°C. Ralstonia eutropha cells at 30°C remained culturable for 3 weeks while culturability at 5°C constantly decreased. The effect of temperature was significantly stronger in a polyhydroxybutyrate-negative mutant. Our data show that accumulated polyhydroxybutyrate has a cold-protective function and can prevent R. eutropha entering the viable but not culturable state.
(© FEMS 2016.)

Nat Chem Biol. 2016 May;12(5):332-8. (PMID: 26974813)
Microbiology. 2015 Sep;161(9):1816-29. (PMID: 26296349)
Microbiology. 2014 Apr;160(Pt 4):711-22. (PMID: 24515609)
Arch Microbiol. 2002 Aug;178(2):85-93. (PMID: 12115053)
Appl Environ Microbiol. 2015 Mar;81(5):1847-58. (PMID: 25548058)
Microb Ecol. 1982 Dec;8(4):313-23. (PMID: 24226049)
Mol Microbiol. 2000 Nov;38(3):626-38. (PMID: 11069685)
Appl Environ Microbiol. 2014 Aug;80(16):4936-46. (PMID: 24907326)
Appl Environ Microbiol. 2015 Dec;81(24):8277-93. (PMID: 26407880)
Environ Microbiol. 2014 Aug;16(8):2357-73. (PMID: 24329995)
J Bacteriol. 2012 Nov;194(21):5909-21. (PMID: 22923598)
J Bacteriol. 2001 Jul;183(14):4217-26. (PMID: 11418562)
Appl Environ Microbiol. 2012 Nov;78(22):7884-90. (PMID: 22941075)
Appl Environ Microbiol. 1988 Aug;54(8):1977-82. (PMID: 16347708)
Chem Commun (Camb). 2016 Feb 11;52(12):2632-5. (PMID: 26750202)
Appl Environ Microbiol. 1993 May;59(5):1560-4. (PMID: 8517752)
Subcell Biochem. 2012;64:343-66. (PMID: 23080259)
PLoS One. 2016 Jan 25;11(1):e0147593. (PMID: 26808070)
J Bacteriol. 2000 Oct;182(20):5916-8. (PMID: 11004196)
Crit Rev Biotechnol. 2016 Dec;36(6):978-991. (PMID: 26329669)
Appl Microbiol Biotechnol. 2014 Feb;98(4):1469-83. (PMID: 24343766)
FEMS Microbiol Rev. 2010 Jul;34(4):415-25. (PMID: 20059548)
Nat Rev Microbiol. 2010 Aug;8(8):578-92. (PMID: 20581859)
J Microbiol. 2005 Feb;43 Spec No:93-100. (PMID: 15765062)
Antonie Van Leeuwenhoek. 1998 Feb;73(2):169-87. (PMID: 9717575)
J Mol Microbiol Biotechnol. 2005;10(2-4):181-96. (PMID: 16645314)
Front Microbiol. 2014 Jun 02;5:258. (PMID: 24917854)
Nature. 1961 Jul 29;191:463-5. (PMID: 13747776)
Nat Biotechnol. 2006 Oct;24(10):1257-62. (PMID: 16964242)

Keywords: VBNC; polyhydroxybutyrate; survival; viable but not culturable

0 (Hydroxybutyrates)

Date Created: 20161105 Date Completed: 20171121 Latest Revision: 20181113

20231215

PMC5175184

10.1093/femsle/fnw249

27810883