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El cultivo del sedimento de 50 mL no mejora sustancialmente el diagnóstico de peritonitis asociada a diálisis. (Spanish)
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- Author(s): Rodríguez-Frausto, Manuel; Medina, Humberto; Macías, Alejandro E.
- Source:
Revista Mexicana de Patologia Clinica; abr-jun2007, Vol. 54 Issue 2, p72-77, 6p- Subject Terms:
- Source:
- Additional Information
- Abstract: Culturing the centrifuged pellet of 50 mL does not improve significantly the diagnosis of dialysis-associated peritonitis. Background: To improve the diagnosis of dialysis-associated peritonitis, culturing the centrifuged pellet of 50 mL has been proposed. Methods: Study of 144 specimens from 144 patients with peritonitis associated to dialysis, without antibiotic treatment. The specimens were processed by three methods: 1) direct plating; 2) culturing the pellet of 50 mL; and 3) inoculating 10 mL in blood-culture bottle (gold standard). The result of the cultures and the identification of the germs were blinded. Results: Culture is blood-culture bottles rendered 92 isolates (63.9%). Of the centrifuged specimens, 73 isolations were obtained (50.7%), with sensitivity of 0.76, and specificity of 0.95. The technique of direct culture in agar plate found 54 isolations (37.5%) with sensitivity of 0.57, and specificity of 0.95. Conclusions: When compared against the gold standard, culturing the sediment of 50 mL is cumbersome and it does not significantly improve the efficiency of culturing the dialysis fluid. [ABSTRACT FROM AUTHOR]
- Abstract: Antecedentes: Para mejorar el diagnóstico de peritonitis asociada a diálisis, se ha propuesto cultivar el sedimento del centrifugado de 50 mL. Métodos: Se incluyeron 144 muestras de 144 pacientes con peritonitis asociada a diálisis sin tratamiento antibiótico previo. La siembra en frasco de hemocultivo fue el estándar de oro. Las muestras se procesaron por tres métodos: 1) siembra de sedimento; 2) siembra directa; 3) siembra en frasco de hemocultivo. El resultado del cultivo y la identificación de los gérmenes fueron cegados. Resultados: La siembra en frasco de hemocultivo produjo 92 aislamientos (63.9%). Del cultivo de sedimento se obtuvieron 73 aislamientos (50.7%), con sensibilidad de 0.76 y especificidad de 0.95. El cultivo directo encontró 54 aislamientos (37.5%), con sensibilidad de 0.57 y especificidad de 0.95. Conclusiones: Cuando se compara con el estándar de oro, el cultivo del sedimento es laborioso y no mejora sustancialmente la eficacia del diagnóstico. [ABSTRACT FROM AUTHOR]
- Abstract: Copyright of Revista Mexicana de Patologia Clinica is the property of Federacion Mexicana de Patologia Clinica y de la Asociacion Latinoamericana de Patologia Clinica and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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