NFATC3 promotes IRF7 transcriptional activity in plasmacy--toid dendritic cells.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Additional Information
    • Source:
      Publisher: Rockefeller University Press Country of Publication: United States NLM ID: 2985109R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1540-9538 (Electronic) Linking ISSN: 00221007 NLM ISO Abbreviation: J Exp Med Subsets: MEDLINE
    • Publication Information:
      Original Publication: New York, NY : Rockefeller University Press
    • Subject Terms:
      Transcription, Genetic*/drug effects; Dendritic Cells/*metabolism ; Interferon Regulatory Factor-7/*genetics ; NFATC Transcription Factors/*metabolism; Animals ; Base Sequence ; CRISPR-Cas Systems/drug effects ; Cell Nucleus/drug effects ; Cell Nucleus/metabolism ; Cells, Cultured ; Dendritic Cells/drug effects ; Gene Knockdown Techniques ; HEK293 Cells ; Humans ; Interferon Regulatory Factor-7/metabolism ; Interferon Type I/genetics ; Interferon Type I/metabolism ; Mice ; NFATC Transcription Factors/chemistry ; Oligodeoxyribonucleotides/pharmacology ; Promoter Regions, Genetic/genetics ; Protein Binding/drug effects ; Protein Binding/genetics ; Protein Domains ; Protein Transport/drug effects ; Signal Transduction/drug effects ; Structure-Activity Relationship
    • Abstract:
      Plasmacytoid dendritic cells (pDCs) rapidly produce large amounts of type 1 interferon (IFN) after Toll-like receptor 7 and 9 engagements. This specialized function of type 1 IFN production is directly linked to the constitutive expression of IRF7, the master transcription factor for type 1 IFN production. However, the IRF7 regulatory network in pDCs remains largely unknown. In this study, we identify that the transcription factor NFATC3 specifically binds to IRF7 and enhances IRF7-mediated IFN production. Furthermore, knockout of NFATC3 greatly reduced the CpG DNA-induced nuclear translocation of IRF7, which resulted in impaired type 1 IFN production in vitro and in vivo. In addition, we found that NFATC3 and IRF7 both bound to type 1 IFN promoters and that the NFAT binding site in IFN promoters was required for IRF7-mediated IFN expression. Collectively, our study shows that the transcription factor NFATC3 binds to IRF7 and functions synergistically to enhance IRF7-mediated IFN expression in pDCs.
      (© 2016 Bao et al.)
    • References:
      Annu Rev Immunol. 1997;15:707-47. (PMID: 9143705)
      J Biol Chem. 1995 Aug 25;270(34):19898-907. (PMID: 7650004)
      Eur J Immunol. 2012 Aug;42(8):1924-31. (PMID: 22706795)
      Protein Cell. 2013 Jan;4(1):40-52. (PMID: 23132256)
      J Exp Med. 2002 Apr 15;195(8):1003-12. (PMID: 11956291)
      J Exp Med. 2005 Jan 17;201(2):181-7. (PMID: 15657288)
      Annu Rev Immunol. 2005;23:275-306. (PMID: 15771572)
      Eur J Immunol. 2012 Mar;42(3):573-9. (PMID: 22488361)
      Clin Immunol Immunopathol. 1996 Sep;80(3 Pt 2):S40-5. (PMID: 8811062)
      J Immunol. 2006 Nov 15;177(10):6667-74. (PMID: 17082579)
      Nat Rev Immunol. 2010 Sep;10(9):645-56. (PMID: 20725108)
      Nature. 2012 Oct 18;490(7420):421-5. (PMID: 22982991)
      Science. 2015 Mar 13;347(6227):aaa2630. (PMID: 25636800)
      Nature. 2009 Jul 9;460(7252):264-8. (PMID: 19525933)
      Immunity. 1998 Jan;8(1):125-34. (PMID: 9462518)
      J Immunol. 2006 Jan 1;176(1):248-55. (PMID: 16365416)
      J Exp Med. 2011 Apr 11;208(4):823-39. (PMID: 21464221)
      Mol Cell Biol. 2012 Mar;32(6):1164-72. (PMID: 22252322)
      Nature. 2006 Apr 13;440(7086):949-53. (PMID: 16612387)
      J Immunol. 2012 Jul 15;189(2):786-92. (PMID: 22706086)
      Immunity. 2010 Dec 14;33(6):863-77. (PMID: 21167755)
      Int J Clin Pract Suppl. 2015 May;(183):43-52. (PMID: 26177348)
      Nature. 2008 Mar 20;452(7185):323-8. (PMID: 18272964)
      Nature. 2005 Apr 7;434(7034):772-7. (PMID: 15800576)
      Nature. 2005 Apr 21;434(7036):1035-40. (PMID: 15815647)
      EMBO J. 1998 Feb 16;17(4):1087-95. (PMID: 9463386)
      Liver Transpl. 2008 Mar;14 (3):292-8. (PMID: 18306331)
      Cell. 2006 Jul 28;126(2):375-87. (PMID: 16873067)
      Immunity. 1998 Jan;8(1):115-24. (PMID: 9462517)
      Immunol Today. 1992 Apr;13(4):136-42. (PMID: 1374612)
      Nat Immunol. 2013 Dec;14(12):1237-46. (PMID: 24185615)
      Cell. 2002 Apr;109 Suppl:S67-79. (PMID: 11983154)
      EJNMMI Res. 2014 Dec;4(1):73. (PMID: 26116130)
      J Immunol. 2011 Nov 1;187(9):4754-63. (PMID: 21940674)
      Nat Immunol. 2002 Jan;3(1):48-54. (PMID: 11740499)
      Pediatr Blood Cancer. 2009 Apr;52(4):525-7. (PMID: 19058202)
      Cell. 2007 Jun 15;129(6):1111-23. (PMID: 17574024)
    • Accession Number:
      0 (CPG-oligonucleotide)
      0 (IRF7 protein, human)
      0 (Interferon Regulatory Factor-7)
      0 (Interferon Type I)
      0 (Irf7 protein, mouse)
      0 (NFATC Transcription Factors)
      0 (Oligodeoxyribonucleotides)
      0 (transcription factor NF-AT c3)
    • Publication Date:
      Date Created: 20161005 Date Completed: 20170731 Latest Revision: 20181113
    • Publication Date:
      20231215
    • Accession Number:
      PMC5068237
    • Accession Number:
      10.1084/jem.20160438
    • Accession Number:
      27697837