The influence of incubation time on adenovirus quantitation in A549 cells by most probable number.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Additional Information
    • Source:
      Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 8005839 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0984 (Electronic) Linking ISSN: 01660934 NLM ISO Abbreviation: J Virol Methods Subsets: MEDLINE
    • Publication Information:
      Original Publication: [Amsterdam] : Elsevier/North-Holland Biomedical Press, 1980-
    • Subject Terms:
      Adenoviridae/*growth & development ; Viral Load/*methods; A549 Cells ; Adenoviridae/radiation effects ; Adenoviruses, Human/growth & development ; Adenoviruses, Human/radiation effects ; Cell Line ; Humans ; Time Factors ; Ultraviolet Rays ; Virus Replication
    • Abstract:
      Cell culture based assays used to detect waterborne viruses typically call for incubating the sample for at least two weeks in order to ensure that all the culturable virus present is detected. Historically, this estimate was based, at least in part, on the length of time used for detecting poliovirus. In this study, we have examined A549 cells infected with human adenovirus type 2, and have found that a three week incubation of virus infected cells results in a higher number of detected viruses by quantal assay than what is seen after two weeks of incubation, with an average 955% increase in Most Probable Number (MPN) from 2 weeks to 3 weeks. This increase suggests that the extended incubation time is essential for accurately estimating viral titer, particularly for slow-growing viruses, UV treated samples, or samples with low titers of virus. In addition, we found that for some UV-treated samples, there was no detectable MPN at 2 weeks, but after 3 weeks, MPN values were obtained. For UV-treated samples, the average increase in MPN from 2 weeks to 3 weeks was 1401%, while untreated samples averaged a change in MPN of 674%, leading us to believe that the UV-damaged viral DNA may be able to be repaired such that viral replication then occurs.
      (Published by Elsevier B.V.)
    • Contributed Indexing:
      Keywords: Adenovirus; Adenovirus disinfection; Adenovirus quantitation; Cell culture; UV treatment; Virus recovery
    • Publication Date:
      Date Created: 20160907 Date Completed: 20170731 Latest Revision: 20171222
    • Publication Date:
      20240829
    • Accession Number:
      10.1016/j.jviromet.2016.09.002
    • Accession Number:
      27596270