A novel Ap2/ERF transcription factor from Stipa purpurea leads to enhanced drought tolerance in Arabidopsis thaliana.

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  • Additional Information
    • Source:
      Publisher: Springer Country of Publication: Germany NLM ID: 9880970 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-203X (Electronic) Linking ISSN: 07217714 NLM ISO Abbreviation: Plant Cell Rep Subsets: MEDLINE
    • Publication Information:
      Original Publication: Berlin ; New York : Springer, 1981-
    • Subject Terms:
      Adaptation, Physiological*/genetics ; Droughts*; Arabidopsis/*genetics ; Arabidopsis/*physiology ; Plant Proteins/*metabolism ; Poaceae/*metabolism ; Transcription Factor AP-2/*metabolism; Amino Acid Sequence ; Arabidopsis/growth & development ; Base Sequence ; Cloning, Molecular ; Electrolytes/metabolism ; Electrophoretic Mobility Shift Assay ; Gene Expression Regulation, Plant ; Genes, Plant ; Phenotype ; Phylogeny ; Plant Proteins/genetics ; Plants, Genetically Modified ; Poaceae/genetics ; Proline/metabolism ; Promoter Regions, Genetic/genetics ; Protein Binding ; Recombinant Fusion Proteins/metabolism ; Seedlings/genetics ; Stress, Physiological/genetics ; Subcellular Fractions/metabolism ; Transcription Factor AP-2/genetics ; Up-Regulation/genetics
    • Abstract:
      Key Message: SpERF1 acts as a positive regulator, contributing to drought stress tolerance in A. thaliana through activating DRE/CRT elements in the promoters of abiotic stress-responsive genes. Stipa purpurea is an endemic perennial grass species in alpine arid and semi-arid meadows on the Qinghai-Xizang Plateau, which is highly tolerant against drought and cold. ERF transcription factors are known to regulate gene expression under abiotic and biotic treatments. Herein, we isolated a full-length ERF gene CDS from S. purpurea named SpERF1, which was induced by drought, cold, and jasmonic acid stresses. Subcellular localization revealed that SpERF1 is a nuclear protein, consistent with its roles as a transcription factor. Overexpression of SpERF1 enhanced drought tolerance of transgenic Arabidopsis thaliana via the activation of DRE/CRT elements in the promoters of abiotic stress-responsive genes. Furthermore, increased accumulation of proline indicated that SpERF1 might be involved in proline synthesis in the transgenic lines, allowing them to have a better buffering capacity and membrane protection under drought stress. This study indicated that SpERF1 might be an attractive target in the genetic engineering for improving stress tolerance in other crops. Moreover, SpERF1 protein function analysis increased our understanding of S. purpurea's ability to adapt to the adverse conditions of the Qinghai-Xizang Plateau.
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    • Contributed Indexing:
      Keywords: Cloning*; Drought*; Gene expression*; SpERF1*; Stipa purpurea*
    • Accession Number:
      0 (Electrolytes)
      0 (Plant Proteins)
      0 (Recombinant Fusion Proteins)
      0 (Transcription Factor AP-2)
      9DLQ4CIU6V (Proline)
    • Publication Date:
      Date Created: 20160723 Date Completed: 20170327 Latest Revision: 20220310
    • Publication Date:
      20231215
    • Accession Number:
      10.1007/s00299-016-2030-y
    • Accession Number:
      27443644