Prothrombin and fibrinogen carbonylation: How that can affect the blood clotting.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read Online Read More Add to Saved list
  • Author(s): Harutyunyan HA;Harutyunyan HA;Harutyunyan HA
  • Source:
    Redox report : communications in free radical research [Redox Rep] 2017 Jul; Vol. 22 (4), pp. 160-165. Date of Electronic Publication: 2016 Jun 27.
  • Publication Type:
    Journal Article
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Taylor & Francis Country of Publication: England NLM ID: 9511366 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1743-2928 (Electronic) Linking ISSN: 13510002 NLM ISO Abbreviation: Redox Rep Subsets: MEDLINE
    • Publication Information:
      Publication: 2016- : Abingdon : Taylor & Francis
      Original Publication: Edinburgh ; New York : Churchill Livingstone, c1994-
    • Subject Terms:
      Blood Coagulation/*physiology ; Fibrinogen/*metabolism ; Prothrombin/*metabolism; Animals ; Hydrogen Peroxide/metabolism ; Male ; Protein Carbonylation ; Rats ; Reactive Oxygen Species/metabolism
    • Abstract:
      Objectives: The aim of the work was the development of a simple method for measuring the plasma prothrombin carbonylation and the study the impact of prothrombin and fibrinogen oxidation on the rate of plasma clotting.
      Methods: A new method was based on the ability of prothrombin to be adsorbed by the barium sulfate. It consists of four steps: prothrombin mixing with the water suspension of BaSO 4 ; reaction of 2,4-dinitrophenylhydrazine with the BaSO 4 -bound prothrombin; desorption of prothrombin-2,4-dinitrophenylhydrazone complex from BaSO 4 in an alkaline medium; neutralization and reading of the optical absorbance of the complex (λ = 370 nm). The prothrombin/fibrinogen carbonylation and plasma clotting rate in vitro in the presence of reactive oxygen species (ROS)-generating agents (0.05-0.8 mM Fe 2+ /H 2 O 2 ) were monitored.
      Results: The plasma volume required for measurement of carbonylated prothrombin was 0.4 ml. High level of linearity and reproducibility was observed (r = 0.9995, P = 0.0005 - for the protein; r = 0.9971, P = 0.0029 - for carbonyls). In the intact rats, the concentration of blood plasma prothrombin was 0.355 ± 0.009 mg/ml, and that of carbonyls was 4.94 ± 0.09 nmol/mg.
      Discussion: Prothrombin and plasma clotting rate was not affected by low concentrations of ROS (0.05-0.2 mM Fe 2+ /H 2 O 2 ). The fibrinogen was susceptible to ROS-related effect over all the used range of concentration (0.05-0.8 mM Fe 2+ /H 2 O 2 ). Carbonylation of fibrinogen did not affect the plasma clotting activity at low ROS concentration (0.05-0.2 mM Fe 2+ /H 2 O 2 ), however it retarded the clotting at higher ROS (0.2-0.8 mM Fe 2+ /H 2 O 2 ).
    • References:
      Clin Appl Thromb Hemost. 2011 Jun;17(3):259-63. (PMID: 20530059)
      Nature. 1970 Aug 15;227(5259):680-5. (PMID: 5432063)
      Free Radic Res. 2014 Oct;48(10):1145-62. (PMID: 25072785)
      Blood Rev. 2007 May;21(3):131-42. (PMID: 17208341)
      J Biol Chem. 1967 Dec 10;242(23):5611-6. (PMID: 12325379)
      Biochem J. 1997 May 15;324 ( Pt 1):1-18. (PMID: 9164834)
      Biochem J. 1956 Oct;64(2):286-96. (PMID: 13363839)
      J Biol Chem. 1973 Nov 25;248(22):7729-41. (PMID: 4796071)
      Free Radic Biol Med. 2005 Nov 15;39(10):1362-7. (PMID: 16257645)
      Redox Rep. 2009;14(4):159-66. (PMID: 19695123)
      Biosci Biotechnol Biochem. 2001 Jun;65(6):1315-20. (PMID: 11471730)
      Methods Enzymol. 1990;186:464-78. (PMID: 1978225)
      Free Radic Biol Med. 2002 May 1;32(9):797-803. (PMID: 11978481)
      Bull Exp Biol Med. 2007 Sep;144(3):397-407. (PMID: 18457045)
      Clin Chim Acta. 2003 Mar;329(1-2):23-38. (PMID: 12589963)
      Drug Metab Rev. 2000 Aug-Nov;32(3-4):307-26. (PMID: 11139131)
      Biochem Biophys Res Commun. 1971 Jun 4;43(5):1040-8. (PMID: 5568175)
      J Thromb Thrombolysis. 1998;5(1):9-14. (PMID: 10608044)
      Free Radic Biol Med. 1993 Oct;15(4):435-45. (PMID: 8225025)
      Free Radic Res. 2000 Nov;33 Suppl:S99-108. (PMID: 11191280)
    • Contributed Indexing:
      Keywords: Blood clotting; Fibrinogen; Oxidation; Protein carbonyls; Prothrombin
    • Accession Number:
      0 (Reactive Oxygen Species)
      9001-26-7 (Prothrombin)
      9001-32-5 (Fibrinogen)
      BBX060AN9V (Hydrogen Peroxide)
    • Publication Date:
      Date Created: 20160629 Date Completed: 20180309 Latest Revision: 20231019
    • Publication Date:
      20231019
    • Accession Number:
      PMC6837719
    • Accession Number:
      10.1080/13510002.2016.1200289
    • Accession Number:
      27349300