Temporal Profiles of Stress Protein Inductions after Focal Transient Ischemia in Mice Brain.

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  • Additional Information
    • Source:
      Publisher: Saunders Country of Publication: United States NLM ID: 9111633 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-8511 (Electronic) Linking ISSN: 10523057 NLM ISO Abbreviation: J Stroke Cerebrovasc Dis Subsets: MEDLINE
    • Publication Information:
      Publication: Philadelphia, PA : Saunders
      Original Publication: New York, NY : Demos Publications, [1991-
    • Subject Terms:
      Brain/*metabolism ; Heat-Shock Proteins/*metabolism ; Infarction, Middle Cerebral Artery/*metabolism ; Ischemic Attack, Transient/*metabolism; Animals ; Brain/pathology ; Disease Models, Animal ; Glutathione/metabolism ; HSC70 Heat-Shock Proteins/metabolism ; HSP72 Heat-Shock Proteins/metabolism ; Hypoxia-Inducible Factor 1, alpha Subunit/metabolism ; Immunohistochemistry ; Infarction, Middle Cerebral Artery/pathology ; Ischemic Attack, Transient/pathology ; Mice, Transgenic ; Time Factors ; Ubiquitin/metabolism
    • Abstract:
      Background: Stress proteins have been found to play important protective roles against ischemic brain injury under hypoxic, oxidative, heat shock, and proteasome stresses.
      Methods: In the present study, we investigated the temporal profiles of the major stress proteins including hypoxia-inducible factor-1α (HIF-1α), glutathione (GSH), heat shock protein 72 (HSP72), constitutive heat shock cognate protein 73 (HSC73), and ubiquitin after 45 minutes of transient middle cerebral artery occlusion (tMCAO) in the mice brain up to 7 days after reperfusion.
      Results: Immunohistochemical analyses of HIF-1α, GSH, HSP72, and ubiquitin showed little immunoreactivity of neural cells in sham control brain, whereas HSC73 showed a constitutive immunoreactivity. After tMCAO, HSC73 showed the fastest increase at 12 hours in the peri-ischemic area, followed by HIF-1α with a peak at 24 hours, GSH, HSP72, and ubiquitin with a peak at 72 hours. All these stress proteins returned toward the baseline levels until 7 days. In the ischemic core, these stress proteins showed a similar change with less reaction compared to the peri-ischemic area.
      Conclusions: These data showed temporal expressions of HIF-1α, GSH, HSP72, HSC73, and ubiquitin in the mice brain after tMCAO, which might provide a better understanding of neuroprotective mechanisms and novel targets for therapeutic intervention of brain ischemic disease.
      (Copyright © 2016 National Stroke Association. Published by Elsevier Inc. All rights reserved.)
    • Contributed Indexing:
      Keywords: Cerebral ischemia; GSH; HIF-1α; HSC73; HSP72; ubiquitin
    • Accession Number:
      0 (HSC70 Heat-Shock Proteins)
      0 (HSP72 Heat-Shock Proteins)
      0 (Heat-Shock Proteins)
      0 (Hif1a protein, mouse)
      0 (Hspa8 protein, mouse)
      0 (Hypoxia-Inducible Factor 1, alpha Subunit)
      0 (Ubiquitin)
      GAN16C9B8O (Glutathione)
    • Publication Date:
      Date Created: 20160620 Date Completed: 20170410 Latest Revision: 20170410
    • Publication Date:
      20231215
    • Accession Number:
      10.1016/j.jstrokecerebrovasdis.2016.05.031
    • Accession Number:
      27318648