Human DNA polymerase ε is phosphorylated at serine-1940 after DNA damage and interacts with the iron-sulfur complex chaperones CIAO1 and MMS19.

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  • Additional Information
    • Source:
      Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101139138 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1568-7856 (Electronic) Linking ISSN: 15687856 NLM ISO Abbreviation: DNA Repair (Amst) Subsets: MEDLINE
    • Publication Information:
      Original Publication: Amsterdam ; New York : Elsevier, c2002-
    • Subject Terms:
      DNA Repair*; DNA/*metabolism ; DNA Polymerase II/*metabolism ; Metallochaperones/*metabolism ; Protein Subunits/*metabolism ; Transcription Factors/*metabolism; Alanine/metabolism ; Amino Acid Substitution ; Cell Line, Tumor ; Cell Proliferation ; Cell Survival ; DNA/genetics ; DNA Damage ; DNA Polymerase II/genetics ; HEK293 Cells ; Humans ; Iron-Sulfur Proteins/genetics ; Iron-Sulfur Proteins/metabolism ; Metallochaperones/genetics ; Mutation ; Osteoblasts/cytology ; Osteoblasts/metabolism ; Phosphorylation ; Poly-ADP-Ribose Binding Proteins ; Protein Subunits/genetics ; Serine/metabolism ; Transcription Factors/genetics
    • Abstract:
      We describe a dynamic phosphorylation on serine-1940 of the catalytic subunit of human Pol ε, POLE1, following DNA damage. We also describe novel interactions between POLE1 and the iron-sulfur cluster assembly complex CIA proteins CIAO1 and MMS19. We show that serine-1940 is essential for the interaction between POLE1 and MMS19, but not POLE1 and CIAO1. No defect in either proliferation or survival was identified when POLE1 serine-1940 was mutated to alanine in human cells, even following treatment with DNA damaging agents. We conclude that serine-1940 phosphorylation and the interaction between serine-1940 and MMS19 are not essential functions in the C terminal domain of the catalytic subunit of DNA polymerase ε.
      (Copyright © 2016 Elsevier B.V. All rights reserved.)
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    • Grant Information:
      P30 CA047904 United States CA NCI NIH HHS; P50 CA090440 United States CA NCI NIH HHS; R01 CA148644 United States CA NCI NIH HHS; UM1 CA186690 United States CA NCI NIH HHS
    • Contributed Indexing:
      Keywords: CIAO1; DNA damage; DNA polymerase epsilon; MMS19
    • Accession Number:
      0 (CIAO1 protein, human)
      0 (Iron-Sulfur Proteins)
      0 (MMS19 protein, human)
      0 (Metallochaperones)
      0 (Poly-ADP-Ribose Binding Proteins)
      0 (Protein Subunits)
      0 (Transcription Factors)
      452VLY9402 (Serine)
      9007-49-2 (DNA)
      EC 2.7.7.7 (DNA Polymerase II)
      EC 2.7.7.7 (POLE protein, human)
      OF5P57N2ZX (Alanine)
    • Publication Date:
      Date Created: 20160529 Date Completed: 20170515 Latest Revision: 20201209
    • Publication Date:
      20240829
    • Accession Number:
      PMC4917431
    • Accession Number:
      10.1016/j.dnarep.2016.04.007
    • Accession Number:
      27235625