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Study on the interaction between three benzimidazole anthelmintics and eosin Y by high performance liquid chromatography associating with resonance light scattering and its application.
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- Author(s): Pan Z;Pan Z; Peng J; Peng J; Zang X; Zang X; Lei G; Lei G; He Y; He Y; Liu D; Liu D
- Source:
Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2016 Jul; Vol. 408 (19), pp. 5247-59. Date of Electronic Publication: 2016 May 21.- Publication Type:
Comparative Study; Evaluation Study; Journal Article- Language:
English - Source:
- Additional Information
- Source: Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 101134327 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1618-2650 (Electronic) Linking ISSN: 16182642 NLM ISO Abbreviation: Anal Bioanal Chem Subsets: MEDLINE
- Publication Information: Original Publication: Heidelberg : Springer-Verlag, 2002-
- Subject Terms: Anthelmintics/*chemistry ; Benzimidazoles/*chemistry ; Chromatography, High Pressure Liquid/*methods ; Eosine Yellowish-(YS)/*chemistry ; Refractometry/*methods ; Urine/*chemistry; Anthelmintics/analysis ; Benzimidazoles/analysis ; Binding Sites ; Fluorescent Dyes/chemistry ; Humans ; Hydrogen-Ion Concentration ; Light ; Protein Binding ; Protein Interaction Mapping ; Reproducibility of Results ; Scattering, Radiation ; Sensitivity and Specificity
- Abstract: A novel, highly selective, and sensitive resonance light scattering (RLS) detection approach coupled with high performance liquid chromatography (HPLC) was researched and developed for the synchronous analysis of three kinds of benzimidazole anthelmintics, including mebendazole (MBZ), albendazole (ABZ), and fenbendazole (FBZ) for the first time. In the pH range of 3.5-3.7 Britton-Robinson buffer medium, three kinds of anthelmintics, which were separated by HPLC, reacted with eosin Y (EY) to form 1:1 ion-association complexes, resulting in significantly enhanced RLS signals and the maximum peak located at 335 nm. The enhanced RLS intensity was in proportion to the MBZ, ABZ, and FBZ concentration in the range 0.2-25, 0.2-23, and 0.15-20 μg/mL, respectively. The limit of detection was in the range of 0.064-0.16 μg/mL. In addition, human urine was determined to validate the proposed method by spiked samples and real urine samples. Satisfactory results were obtained by HPLC-RLS method. Graphical Abstract The diagram mechanism of generating resonance between emitted light and scattered light.
- Contributed Indexing: Keywords: Benzimidazole anthelmintics; Eosin Y; High performance liquid chromatography; Resonance light scattering
- Accession Number: 0 (Anthelmintics)
0 (Benzimidazoles)
0 (Fluorescent Dyes)
E24GX49LD8 (benzimidazole)
TDQ283MPCW (Eosine Yellowish-(YS)) - Publication Date: Date Created: 20160523 Date Completed: 20180117 Latest Revision: 20191210
- Publication Date: 20240628
- Accession Number: 10.1007/s00216-016-9619-4
- Accession Number: 27209593
- Source:
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