Characterization of a Novel Polerovirus Infecting Maize in China.

Publisher: MDPI Country of Publication: Switzerland NLM ID: 101509722 Publication Model: Electronic Cited Medium: Internet ISSN: 1999-4915 (Electronic) Linking ISSN: 19994915 NLM ISO Abbreviation: Viruses Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI

Genetic Variation* ; Phylogeny*; Luteoviridae/*classification ; Luteoviridae/*isolation & purification ; Plant Diseases/*virology ; Zea mays/*virology; China ; Cloning, Molecular ; Cluster Analysis ; Gene Silencing ; Genome, Viral ; High-Throughput Nucleotide Sequencing ; Host-Pathogen Interactions ; Luteoviridae/genetics ; Plant Leaves/virology ; Reverse Genetics ; Sequence Analysis, DNA ; Sequence Homology ; Nicotiana/genetics ; Nicotiana/virology

A novel virus, tentatively named Maize Yellow Mosaic Virus (MaYMV), was identified from the field-grown maize plants showing yellow mosaic symptoms on the leaves collected from the Yunnan Province of China by the deep sequencing of small RNAs. The complete 5642 nucleotide (nt)-long genome of the MaYMV shared the highest nucleotide sequence identity (73%) to Maize Yellow Dwarf Virus-RMV. Sequence comparisons and phylogenetic analyses suggested that MaYMV represents a new member of the genus Polerovirus in the family Luteoviridae. Furthermore, the P0 protein encoded by MaYMV was demonstrated to inhibit both local and systemic RNA silencing by co-infiltration assays using transgenic Nicotiana benthamiana line 16c carrying the GFP reporter gene, which further supported the identification of a new polerovirus. The biologically-active cDNA clone of MaYMV was generated by inserting the full-length cDNA of MaYMV into the binary vector pCB301. RT-PCR and Northern blot analyses showed that this clone was systemically infectious upon agro-inoculation into N. benthamiana. Subsequently, 13 different isolates of MaYMV from field-grown maize plants in different geographical locations of Yunnan and Guizhou provinces of China were sequenced. Analyses of their molecular variation indicate that the 3' half of P3-P5 read-through protein coding region was the most variable, whereas the coat protein- (CP-) and movement protein- (MP-)coding regions were the most conserved.

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Keywords: Maize yellow mosaic virus; deep sequencing; infectious clone; molecular variation; sRNA; suppressor

Date Created: 20160503 Date Completed: 20170913 Latest Revision: 20231213

20231215

PMC4885075

10.3390/v8050120

27136578