Characteristics of three-dimensional prospectively isolated mouse bone marrow mesenchymal stem/stromal cell aggregates on nanoculture plates.

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  • Additional Information
    • Source:
      Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 0417625 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-0878 (Electronic) Linking ISSN: 0302766X NLM ISO Abbreviation: Cell Tissue Res Subsets: MEDLINE
    • Publication Information:
      Original Publication: Berlin, New York, Springer-Verlag.
    • Subject Terms:
      Imaging, Three-Dimensional*; Bone Marrow Cells/*cytology ; Cell Culture Techniques/*methods ; Cell Separation/*methods ; Mesenchymal Stem Cells/*cytology ; Nanotechnology/*methods; Animals ; Biomarkers/metabolism ; Bone Marrow Cells/drug effects ; Bone Marrow Cells/metabolism ; Cell Aggregation/drug effects ; Cell Lineage/drug effects ; Cell Proliferation/drug effects ; Cell Shape/drug effects ; Cell Size/drug effects ; Cell Survival/drug effects ; Cell Tracking ; Cells, Cultured ; Culture Media, Conditioned/pharmacology ; Gene Expression Regulation/drug effects ; Male ; Mesenchymal Stem Cells/drug effects ; Mesenchymal Stem Cells/metabolism ; Mice, Inbred C57BL ; Neovascularization, Physiologic/drug effects
    • Abstract:
      Three-dimensional (3-D) aggregate culturing is useful for investigating the functional properties of mesenchymal stem/stromal cells (MSCs). For 3-D MSC analysis, however, pre-expansion of MSCs with two-dimensional (2-D) monolayer culturing must first be performed, which might abolish their endogenous properties. To avoid the need for 2-D expansion, we used prospectively isolated mouse bone marrow (BM)-MSCs and examined the differences in the biological properties of 2-D and 3-D MSC cultures. The BM-MSCs self-assembled into aggregates on nanoculture plates (NCP) that have nanoimprinted patterns with a low-cellular binding texture. The 3-D MSCs proliferated at the same rate as 2-D-cultured cells by only diffusion culture and secreted higher levels of pro-angiogenic factors such as vascular endothelial growth factor and hepatocyte growth factor (HGF). Conditioned medium from 3-D MSC cultures promoted more capillary formation than that of 2-D MSCs in an in vitro tube formation assay. Matrigel-implanted 3-D MSC aggregates tended to induce angiogenesis in host mice. The 3-D culturing on NCP induced alpha-fetoprotein (AFP) expression in MSCs without the application of AFP- or endodermal-inducible factors, possibly via an HGF-autocrine mechanism, and maintained their differentiation ability for adipocytes, osteocytes, and chondrocytes. Prospectively isolated mouse BM-MSCs expressed low/negative stemness-related genes including Oct3/4, Nanog, and Sox2, which were not enhanced by NCP-based 3-D culturing, suggesting that some of these cells differentiate into meso-endodermal layer cells. Culturing of prospectively isolated MSCs on NCP in 3-D allows the analysis of the biological properties of more closely endogenous BM-MSCs and might contribute to tissue engineering and repair.
    • Contributed Indexing:
      Keywords: 3-D aggregate culture; Alpha-fetoprotein; Mouse bone marrow mesenchymal stem/stromal cells; Nanoculture plate; Tissue engineering
    • Accession Number:
      0 (Biomarkers)
      0 (Culture Media, Conditioned)
    • Publication Date:
      Date Created: 20160422 Date Completed: 20170516 Latest Revision: 20181202
    • Publication Date:
      20240829
    • Accession Number:
      10.1007/s00441-016-2405-y
    • Accession Number:
      27100525