Gas chromatography coupled with mass spectrometric characterization of Curcuma longa: Protection against pathogenic microbes and lipid peroxidation in rat's tissue homogenate.

Publisher: Faculty of Pharmacy, University of Karachi Country of Publication: Pakistan NLM ID: 9426356 Publication Model: Print Cited Medium: Print ISSN: 1011-601X (Print) Linking ISSN: 1011601X NLM ISO Abbreviation: Pak J Pharm Sci Subsets: MEDLINE

Original Publication: Karachi : Faculty of Pharmacy, University of Karachi, [1988-

Gas Chromatography-Mass Spectrometry*; Anti-Infective Agents/*pharmacology ; Antioxidants/*pharmacology ; Curcuma/*chemistry ; Lipid Peroxidation/*drug effects ; Oils, Volatile/*pharmacology ; Oxidative Stress/*drug effects ; Plant Extracts/*pharmacology ; Plant Oils/*pharmacology; Anti-Infective Agents/isolation & purification ; Antioxidants/isolation & purification ; Bacteria/drug effects ; Bacteria/growth & development ; Brain/drug effects ; Brain/metabolism ; Candida albicans/drug effects ; Candida albicans/growth & development ; Disk Diffusion Antimicrobial Tests ; Dose-Response Relationship, Drug ; Ethanol/chemistry ; Kidney/drug effects ; Kidney/metabolism ; Liver/drug effects ; Liver/metabolism ; Malondialdehyde/metabolism ; Nitroprusside/pharmacology ; Oils, Volatile/isolation & purification ; Phytotherapy ; Plant Extracts/isolation & purification ; Plant Oils/isolation & purification ; Plants, Medicinal ; Solvents/chemistry ; Spectrophotometry, Atomic ; Thiobarbituric Acid Reactive Substances/metabolism

The present study was designed to investigate the mineral content and antimicrobial activity of Curcuma Longa extracts and its essential oil. We also determined the lipid peroxidation inhibition activity of the ethanolic extract against sodium nitroprusside (SNP) induced thiobarbituric acid reactive species (TBARS) formation in rat's brain, kidney and liver homogenates. Major constituents of essential oil identified by gas chromatography and mass spectrometry (GCMS) were beta-sesquiphellandrene (38.69%), alpha-curcumene (18.44%) and p-mentha-1,4 (8)-diene (16.29%). Atomic absorption spectroscopy (AAS) was used for the quantitative estimation of Calcium (Ca), Magnesium (Mg), Iron (Fe), Copper (Cu), Zinc (Zn), Chromium (Cr), Nickel (Ni) and Manganese (Mn). The extract showed highest Mg (49.4 mg/l) concentration followed by Ca (35.42 mg/l) and Fe (1.27 mg/l). Our data revealed that the ethanolic extract of Curcuma Longa at 1-10 mg/kg significantly inhibited TBARS production in all tested homogenates. Crude extracts and essential oil were tested against three gram positive bacteria i.e. Bacillus subtilis, Bacillus atrophoeus, Staphylococcus aureus, six gram negative bacteria i.e. Escherichia coli, Klebsiella pneumonias, Salmonella typhi, Pseudomonas aeruginosa, Erwinia carotovora, Agrobacterium tumefaciens and one fungal strain namely Candida albicans by disc diffusion assay. Essential oil showed highest anti-microbial activity as compared to the crude extracts. The present study confirms the significant antimicrobial and antioxidant potential of the studied plant, which can be considered as a diet supplement for a variety of oxidative stress induced or infectious diseases.

0 (Anti-Infective Agents)
0 (Antioxidants)
0 (Oils, Volatile)
0 (Plant Extracts)
0 (Plant Oils)
0 (Solvents)
0 (Thiobarbituric Acid Reactive Substances)
169D1260KM (Nitroprusside)
3K9958V90M (Ethanol)
4Y8F71G49Q (Malondialdehyde)

Date Created: 20160419 Date Completed: 20160630 Latest Revision: 20160418

20240829

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