[MASS-SPECTROMETRY IN MICROBIOLOGICAL PRACTICE OF SCIENTIFIC CENTRE OF OBSTETRICS, GYNECOLOGY AND PERINATOLOGY].

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Additional Information
    • Source:
      Publisher: FBUN T︠S︡NII Ėpidemiologii Rospotrebnadzora Country of Publication: Russia (Federation) NLM ID: 0415217 Publication Model: Print Cited Medium: Print ISSN: 0372-9311 (Print) Linking ISSN: 03729311 NLM ISO Abbreviation: Zh Mikrobiol Epidemiol Immunobiol Subsets: MEDLINE
    • Publication Information:
      Publication: <2018, no. 4 (ii︠u︡lʹ-avgust)- >: Moskva : FBUN T︠S︡NII Ėpidemiologii Rospotrebnadzora
      Original Publication: Moskva : Izd-vo "Medit︠s︡ina"
    • Subject Terms:
      Academic Medical Centers/*methods ; Bacteria/*classification ; Bacterial Typing Techniques/*instrumentation ; Fungi/*classification ; Mycological Typing Techniques/*instrumentation ; RNA, Ribosomal/*genetics; Bacteria/genetics ; Bacteria/isolation & purification ; Female ; Fungi/genetics ; Fungi/isolation & purification ; Gynecology ; Humans ; Infant, Newborn ; Obstetrics ; Perinatology ; Pregnancy ; RNA, Ribosomal/isolation & purification ; Sequence Analysis, RNA ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Vagina/microbiology
    • Abstract:
      Aim: Comparative evaluation of species identification of microorganisms by MALDI-TOF mass-spectrometry and automatic biochemical analyzer VITEK2 Compact 30.
      Materials and Methods: Species identification of 18,400 isolates of microorganisms (staphylococci, streptococci, enterococci, enterobacteria, nonfermenting gram-negative bacteria, lactobacilli, anaerobes, yeast fungi, neisseriae), isolated from vagina of pregnant and non-pregnant women and from newborns, was carried out. Identification of the isolated microorganisms was carried out by automatic bacteriologic analyzer VITEK2 Compact30 (BioMerieuX, France) and MALDI-TOF-MS analysis method on AutofleXIII (Bruker Daltonics, Germany) mass-spectrometer.
      Results: Comparative identification of 2005 isolates of microorganisms was carried out. Sequencing of ribosomal RNA was used as a reference method. Authenticity of species identification my MALDI-TOF-MS analysis method was: for staphylococci (95.8%), enterococci (97.5%), enterobacteria (98.4%), nonfermenting gram-negative bacteria (93.6%), β-hemolytiC staphylococci (93.8%), lactobacilli (92.8%), yeast fungi (99.9%).
      Conclusion: Introduction of MALDI-TOF-MS analysis technology into practical work of microbiological laboratories exceeds previously used methods of microbiological testing in terms of speed; cost and authenticity of identification of a wide spectrum of microorganisms.
    • Accession Number:
      0 (RNA, Ribosomal)
    • Publication Date:
      Date Created: 20160401 Date Completed: 20160408 Latest Revision: 20200716
    • Publication Date:
      20250114
    • Accession Number:
      27029117