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Mutations in lysX as the new and reliable markers for tuberculosis Beijing and modern Beijing strains.
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- Additional Information
- Source:
Publisher: Churchill Livingstone Country of Publication: Scotland NLM ID: 100971555 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-281X (Electronic) Linking ISSN: 14729792 NLM ISO Abbreviation: Tuberculosis (Edinb) Subsets: MEDLINE
- Publication Information:
Original Publication: Edinburgh, New York : Churchill Livingstone, c2001-
- Subject Terms:
- Abstract:
Genotyping results and DNA sequencing analysis of 235 Mycobacterium tuberculosis (M. tuberculosis) isolates from China indicated that mutations at codon 995 (Pro CCG to Pro CCA) and 701 (Ile ATT to Thr ACT) in lysX gene (Rv1640c), are specific markers for Beijing and modern Beijing strains, respectively. This observation was also confirmed by 24 genomes of M. tuberculosis strains from other countries. Moreover, a simple and fast multiplex allele-specific PCR (MAS-PCR) method for detecting mutations at codon 995 and 701 in lysX has been established and used to screen 235 DNA samples obtained from M. tuberculosis isolates. In all cases, Beijing and modern Beijing strains were identified correctly.
(Copyright © 2016 Elsevier Ltd. All rights reserved.)
- Contributed Indexing:
Keywords: Beijing genotype; Genotype; Marker; Modern Beijing subtype; Multiplex allele-specific PCR; Mycobacterium tuberculosis
- Accession Number:
0 (Bacterial Proteins)
0 (Codon)
0 (Genetic Markers)
EC 6.1.1.6 (Lysine-tRNA Ligase)
EC 6.1.1.6 (lysX protein, Mycobacterium tuberculosis)
- Publication Date:
Date Created: 20160317 Date Completed: 20161220 Latest Revision: 20161230
- Publication Date:
20221213
- Accession Number:
10.1016/j.tube.2015.12.006
- Accession Number:
26980493
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