The D-amino acid transport by the invertebrate SLC6 transporters KAAT1 and CAATCH1 from Manduca sexta.

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  • Additional Information
    • Source:
      Publisher: published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society Country of Publication: United States NLM ID: 101607800 Publication Model: Print Cited Medium: Print ISSN: 2051-817X (Print) Linking ISSN: 2051817X NLM ISO Abbreviation: Physiol Rep Subsets: MEDLINE
    • Publication Information:
      Original Publication: [Malden MA] : published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society, 2013-
    • Subject Terms:
      Amino Acid Transport Systems, Neutral/*metabolism ; Amino Acids/*metabolism ; Carrier Proteins/*metabolism ; Insect Proteins/*metabolism ; Manduca/*metabolism ; Membrane Proteins/*metabolism ; Protein Transport/*physiology; Animals ; Isomerism ; Patch-Clamp Techniques
    • Abstract:
      The ability of the SLC6 family members, the insect neutral amino acid cotransporter KAAT1(K(+)-coupled amino acid transporter 1) and its homologous CAATCH1(cation anion activated amino acid transporter/channel), to transport D-amino acids has been investigated through heterologous expression in Xenopus laevis oocytes and electrophysiological techniques. In the presence of D-isomers of leucine, serine, and proline, the msKAAT1 generates inward, transport-associated, currents with variable relative potencies, depending on the driving ion Na(+) or K(+). Higher concentrations of D-leucine (≥1 mmol/L) give rise to an anomalous response that suggests the existence of a second binding site with inhibitory action on the transport process. msCAATCH1 is also able to transport the D-amino acids tested, including D-leucine, whereas L-leucine acts as a blocker. A similar behavior is exhibited by the KAAT1 mutant S308T, confirming the relevance of the residue in this position in L-leucine binding and the different interaction of D-leucine with residues involved in transport mechanism. D-leucine and D-serine on various vertebrate orthologs B(0)AT1 (SLC6A19) elicited only a very small current and singular behavior was not observed, indicating that it is specific of the insect neutral amino acid transporters. These findings highlight the relevance of D-amino acid absorption in the insect nutrition and metabolism and may provide new evidences in the molecular transport mechanism of SLC6 family.
      (© 2016 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.)
    • References:
      Nature. 2005 Sep 8;437(7056):215-23. (PMID: 16041361)
      J Biol Chem. 2008 Aug 8;283(32):22244-56. (PMID: 18544534)
      J Agric Food Chem. 1999 Sep;47(9):3457-79. (PMID: 10552672)
      J Nutr. 1966 Sep;90(1):31-9. (PMID: 5918848)
      Proc Natl Acad Sci U S A. 1999 Jan 19;96(2):721-5. (PMID: 9892700)
      Physiol Rep. 2016 Feb;4(4):. (PMID: 26884475)
      Nucl Med Biol. 2005 Nov;32(8):793-7. (PMID: 16253803)
      Membr Biochem. 1982;4(4):271-82. (PMID: 7176933)
      FEBS J. 2008 Jul;275(14):3514-26. (PMID: 18564180)
      J Insect Physiol. 2012 Apr;58(4):433-49. (PMID: 22230793)
      Biochem Biophys Res Commun. 2002 Feb 22;291(2):291-5. (PMID: 11846403)
      Front Neurosci. 2014 Nov 14;8:365. (PMID: 25452710)
      Insect Biochem Mol Biol. 2008 Oct;38(10):923-31. (PMID: 18718864)
      Biochim Biophys Acta. 2012 Jul;1818(7):1737-44. (PMID: 22402268)
      J Exp Biol. 2006 Aug;209(Pt 16):3183-98. (PMID: 16888066)
      Proc Natl Acad Sci U S A. 1998 Apr 28;95(9):5395-400. (PMID: 9560287)
      Proc Natl Acad Sci U S A. 2005 Feb 1;102(5):1360-5. (PMID: 15665107)
      Pflugers Arch. 1999 Nov;438(6):788-96. (PMID: 10591067)
      Annu Rev Biochem. 1997;66:337-45. (PMID: 9242910)
      Insect Mol Biol. 2009 Jun;18(3):265-79. (PMID: 19389142)
      Biochem Biophys Res Commun. 2005 Nov 25;337(3):892-900. (PMID: 16226721)
      Comp Biochem Physiol A Mol Integr Physiol. 2013 Oct;166(2):285-92. (PMID: 23827520)
      Chem Biodivers. 2010 Jun;7(6):1491-530. (PMID: 20564567)
      Biochem Soc Trans. 2013 Dec;41(6):1551-6. (PMID: 24256253)
      J Biol Chem. 2000 Aug 11;275(32):24518-26. (PMID: 10829035)
      Proc Natl Acad Sci U S A. 2000 Apr 25;97(9):4926-31. (PMID: 10781100)
      J Physiol. 1999 Mar 15;515 ( Pt 3):729-42. (PMID: 10066900)
      Biochim Biophys Acta. 2000 Jan 10;1495(1):34-9. (PMID: 10634930)
      J Physiol. 2007 Jun 15;581(Pt 3):899-913. (PMID: 17412764)
      Am J Physiol Cell Physiol. 2007 Apr;292(4):C1379-87. (PMID: 17135296)
      Annu Rev Nutr. 1987;7:209-25. (PMID: 3300733)
      Science. 1969 Apr 11;164(3876):142-9. (PMID: 5774186)
      Am J Physiol Cell Physiol. 2004 Sep;287(3):C754-61. (PMID: 15140745)
      Amino Acids. 2010 May;38(5):1561-9. (PMID: 19890700)
      Proc Natl Acad Sci U S A. 1999 Nov 9;96(23):13409-14. (PMID: 10557334)
      Biochem Soc Trans. 2005 Feb;33(Pt 1):233-6. (PMID: 15667315)
    • Contributed Indexing:
      Keywords: D‐amino acids; SLC6; Xenopus oocytes; voltage clamp
      Note: Original DateCompleted: 20160217
    • Accession Number:
      0 (Amino Acid Transport Systems, Neutral)
      0 (Amino Acids)
      0 (CAATCH1 protein, insect)
      0 (Carrier Proteins)
      0 (Insect Proteins)
      0 (KAAT1 protein, insect)
      0 (Membrane Proteins)
    • Publication Date:
      Date Created: 20160218 Date Completed: 20160907 Latest Revision: 20210109
    • Publication Date:
      20231215
    • Accession Number:
      PMC4759042
    • Accession Number:
      10.14814/phy2.12691
    • Accession Number:
      26884475