Epidermis-Derived Semaphorin Promotes Dendrite Self-Avoidance by Regulating Dendrite-Substrate Adhesion in Drosophila Sensory Neurons.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Additional Information
    • Source:
      Publisher: Cell Press Country of Publication: United States NLM ID: 8809320 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1097-4199 (Electronic) Linking ISSN: 08966273 NLM ISO Abbreviation: Neuron Subsets: MEDLINE
    • Publication Information:
      Original Publication: [Cambridge, Mass. : Cell Press, c1988-
    • Subject Terms:
      Dendrites/*physiology ; Drosophila Proteins/*metabolism ; Epidermis/*physiology ; Focal Adhesion Kinase 1/*metabolism ; Gene Expression Regulation, Developmental/*genetics ; Semaphorins/*metabolism ; Sensory Receptor Cells/*cytology; Animals ; Animals, Genetically Modified ; Cell Communication ; Drosophila ; Drosophila Proteins/genetics ; Focal Adhesion Kinase 1/genetics ; Green Fluorescent Proteins/genetics ; Green Fluorescent Proteins/metabolism ; Immunoprecipitation ; Larva ; Mechanistic Target of Rapamycin Complex 2 ; Molecular Biology ; Multiprotein Complexes/metabolism ; Mutation/genetics ; Nerve Tissue Proteins/genetics ; Nerve Tissue Proteins/metabolism ; Receptors, Cell Surface/genetics ; Receptors, Cell Surface/metabolism ; Semaphorins/genetics ; TOR Serine-Threonine Kinases/metabolism ; Transfection
    • Abstract:
      Precise patterning of dendritic arbors is critical for the wiring and function of neural circuits. Dendrite-extracellular matrix (ECM) adhesion ensures that the dendrites of Drosophila dendritic arborization (da) sensory neurons are properly restricted in a 2D space, and thereby facilitates contact-mediated dendritic self-avoidance and tiling. However, the mechanisms regulating dendrite-ECM adhesion in vivo are poorly understood. Here, we show that mutations in the semaphorin ligand sema-2b lead to a dramatic increase in self-crossing of dendrites due to defects in dendrite-ECM adhesion, resulting in a failure to confine dendrites to a 2D plane. Furthermore, we find that Sema-2b is secreted from the epidermis and signals through the Plexin B receptor in neighboring neurons. Importantly, we find that Sema-2b/PlexB genetically and physically interacts with TORC2 complex, Tricornered (Trc) kinase, and integrins. These results reveal a novel role for semaphorins in dendrite patterning and illustrate how epidermal-derived cues regulate neural circuit assembly.
      (Copyright © 2016 Elsevier Inc. All rights reserved.)
    • References:
      Nat Rev Neurosci. 2012 Sep;13(9):605-18. (PMID: 22895477)
      PLoS One. 2011;6(5):e20108. (PMID: 21655286)
      EMBO Rep. 2004 Apr;5(4):356-61. (PMID: 15060572)
      Mol Cell Neurosci. 2012 May;50(1):10-20. (PMID: 22465229)
      Genes Dev. 2007 Apr 15;21(8):956-72. (PMID: 17437999)
      Science. 2013 Nov 1;342(6158):1241974. (PMID: 24179230)
      Cell. 2013 Oct 10;155(2):308-20. (PMID: 24120132)
      Cell. 2004 Oct 15;119(2):245-56. (PMID: 15479641)
      Cell. 2007 May 4;129(3):593-604. (PMID: 17482551)
      Elife. 2015 Mar 12;4:. (PMID: 25764303)
      Nature. 2003 Jul 24;424(6947):391-7. (PMID: 12879061)
      Neuron. 1999 Mar;22(3):451-61. (PMID: 10197526)
      Neuron. 2011 Aug 11;71(3):460-73. (PMID: 21835343)
      Neuron. 2011 Dec 8;72(5):734-47. (PMID: 22153371)
      Nat Rev Neurosci. 2004 Oct;5(10):747-57. (PMID: 15378035)
      Nat Methods. 2006 Dec;3(12):995-1000. (PMID: 17072308)
      Nat Rev Mol Cell Biol. 2006 Apr;7(4):253-64. (PMID: 16607288)
      Nature. 2011 Feb 10;470(7333):259-63. (PMID: 21270798)
      Neuron. 2004 Oct 14;44(2):239-49. (PMID: 15473964)
      Cereb Cortex. 2000 Oct;10(10):981-91. (PMID: 11007549)
      Neuron. 2001 Oct 11;32(1):53-62. (PMID: 11604138)
      PLoS Biol. 2009 Jun 16;7(6):e1000135. (PMID: 19547742)
      Development. 2013 Aug;140(16):3297-302. (PMID: 23900538)
      Development. 2002 Jun;129(12):2867-78. (PMID: 12050135)
      Neuron. 2007 May 3;54(3):403-16. (PMID: 17481394)
      Curr Opin Neurobiol. 2014 Aug;27:1-7. (PMID: 24598309)
      Development. 2013 Dec;140(23):4657-71. (PMID: 24255095)
      Development. 2015 Jan 1;142(1):162-73. (PMID: 25480915)
      Nature. 2012 Aug 23;488(7412):517-21. (PMID: 22842903)
      Neuron. 2011 Apr 28;70(2):281-98. (PMID: 21521614)
      Development. 2006 Jun;133(11):2125-35. (PMID: 16672342)
      Curr Biol. 2012 Oct 9;22(19):1831-8. (PMID: 22959350)
      Proc Natl Acad Sci U S A. 2011 Jun 7;108(23):9673-8. (PMID: 21606367)
      Neuron. 2012 Mar 22;73(6):1127-42. (PMID: 22445341)
      Cell. 2013 Oct 10;155(2):296-307. (PMID: 24120131)
      Cell. 2010 Oct 29;143(3):343-53. (PMID: 21029858)
      Nat Genet. 2004 Mar;36(3):283-7. (PMID: 14981521)
      Nat Rev Mol Cell Biol. 2005 Oct;6(10):789-800. (PMID: 16314868)
      Neuron. 2001 Oct 11;32(1):39-51. (PMID: 11604137)
      J Cell Biol. 2013 Nov 11;203(3):395-405. (PMID: 24217617)
      Proc Natl Acad Sci U S A. 2007 Mar 20;104(12):5199-204. (PMID: 17360325)
      Genes Dev. 2013 Jan 15;27(2):157-62. (PMID: 23348839)
      Neuron. 2007 May 3;54(3):417-27. (PMID: 17481395)
      J Biol Chem. 2007 Nov 30;282(48):34888-95. (PMID: 17855350)
      Annu Rev Physiol. 2015;77:271-300. (PMID: 25386991)
      Neuron. 2013 May 22;78(4):673-86. (PMID: 23719164)
      Neuron. 2010 Apr 15;66(1):15-36. (PMID: 20399726)
      J Neurosci. 2014 Apr 9;34(15):5342-54. (PMID: 24719112)
      Annu Rev Neurosci. 2013 Jul 8;36:547-68. (PMID: 23841842)
      Genes Dev. 2012 Oct 1;26(19):2222-35. (PMID: 23028146)
      J Embryol Exp Morphol. 1972 Apr;27(2):353-65. (PMID: 4625067)
      Nat Rev Neurosci. 2010 May;11(5):316-28. (PMID: 20404840)
      Neuron. 2012 Jan 12;73(1):64-78. (PMID: 22243747)
      J Neurosci. 2008 Sep 24;28(39):9599-609. (PMID: 18815246)
      Cell. 2013 Apr 25;153(3):722.e1. (PMID: 23622251)
      Curr Biol. 2005 May 10;15(9):804-14. (PMID: 15886097)
      J Biol Chem. 2003 Feb 28;278(9):6710-8. (PMID: 12493777)
      Nat Cell Biol. 2000 Sep;2(9):574-81. (PMID: 10980697)
      Curr Opin Neurobiol. 2014 Aug;27:75-81. (PMID: 24694749)
      Gene. 2014 Dec 15;553(2):69-74. (PMID: 25300255)
      Development. 2007 Jan;134(1):55-64. (PMID: 17164414)
      EMBO J. 2009 Dec 16;28(24):3879-92. (PMID: 19875983)
      PLoS Biol. 2004 Aug;2(8):E239. (PMID: 15269788)
      Neuron. 2012 Jan 12;73(1):79-91. (PMID: 22243748)
      J Neurosci. 2010 Jan 27;30(4):1452-62. (PMID: 20107072)
      Nature. 1982 Jun 24;297(5868):683-5. (PMID: 7088156)
    • Grant Information:
      R37NS040929 United States NS NINDS NIH HHS; R37 NS040929 United States NS NINDS NIH HHS; R01 NS076614 United States NS NINDS NIH HHS; P30 DK098722 United States DK NIDDK NIH HHS; R01 NS040929 United States NS NINDS NIH HHS; United States Howard Hughes Medical Institute
    • Accession Number:
      0 (Drosophila Proteins)
      0 (Multiprotein Complexes)
      0 (Nerve Tissue Proteins)
      0 (Receptors, Cell Surface)
      0 (Semaphorins)
      0 (plexB protein, Drosophila)
      147336-22-9 (Green Fluorescent Proteins)
      EC 2.7.10.2 (Fak protein, Drosophila)
      EC 2.7.10.2 (Focal Adhesion Kinase 1)
      EC 2.7.11.1 (Mechanistic Target of Rapamycin Complex 2)
      EC 2.7.11.1 (TOR Serine-Threonine Kinases)
    • Publication Date:
      Date Created: 20160209 Date Completed: 20160628 Latest Revision: 20240324
    • Publication Date:
      20240324
    • Accession Number:
      PMC4760923
    • Accession Number:
      10.1016/j.neuron.2016.01.020
    • Accession Number:
      26853303