Brain cortex mitochondrial bioenergetics in synaptosomes and non-synaptic mitochondria during aging.

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  • Additional Information
    • Source:
      Publisher: Kluwer Academic/Plenum Publishers Country of Publication: United States NLM ID: 7613461 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-6903 (Electronic) Linking ISSN: 03643190 NLM ISO Abbreviation: Neurochem Res Subsets: MEDLINE
    • Publication Information:
      Publication: 1999- : New York, NY : Kluwer Academic/Plenum Publishers
      Original Publication: New York, Plenum Press
    • Subject Terms:
      Energy Metabolism*; Aging/*metabolism ; Cerebral Cortex/*metabolism ; Mitochondria/*metabolism ; Synaptosomes/*metabolism; Animals ; Calcium/metabolism ; Male ; Membrane Potential, Mitochondrial ; Mice
    • Abstract:
      Alterations in mitochondrial bioenergetics have been associated with brain aging. In order to evaluate the susceptibility of brain cortex synaptosomes and non-synaptic mitochondria to aging-dependent dysfunction, male Swiss mice of 3 or 17 months old were used. Mitochondrial function was evaluated by oxygen consumption, mitochondrial membrane potential and respiratory complexes activity, together with UCP-2 protein expression. Basal respiration and respiration driving proton leak were decreased by 26 and 33 % in synaptosomes from 17-months old mice, but spare respiratory capacity was not modified by aging. Succinate supported state 3 respiratory rate was decreased by 45 % in brain cortex non-synaptic mitochondria from 17-month-old mice, as compared with young animals, but respiratory control was not affected. Synaptosomal mitochondria would be susceptible to undergo calcium-induced depolarization in 17 months-old mice, while non-synaptic mitochondria would not be affected by calcium overload. UCP-2 was significantly up-regulated in both synaptosomal and submitochondrial membranes from 17-months old mice, compared to young animals. UCP-2 upregulation seems to be a possible mechanism by which mitochondria would be resistant to suffer oxidative damage during aging.
    • References:
      Arch Biochem Biophys. 2004 Oct 15;430(2):170-7. (PMID: 15369815)
      Neurochem Res. 2009 Dec;34(12):2102-16. (PMID: 19495970)
      Aging (Albany NY). 2014 Apr;6(4):320-34. (PMID: 24827396)
      FASEB J. 2002 Sep;16(11):1418-20. (PMID: 12205033)
      Neuroscience. 2012 Dec 27;227:55-66. (PMID: 23022213)
      J Neurochem. 2005 Feb;92(3):494-504. (PMID: 15659220)
      J Neural Transm (Vienna). 2006 Nov;113(11):1659-70. (PMID: 16969625)
      Neuroscientist. 2006 Aug;12(4):291-9. (PMID: 16840705)
      Neurochem Res. 2003 Oct;28(10):1433-41. (PMID: 14570388)
      Life Sci. 2012 May 22;90(19-20):808-14. (PMID: 22525373)
      J Neurosci. 2011 Mar 23;31(12):4524-34. (PMID: 21430153)
      Am J Physiol Endocrinol Metab. 2001 Mar;280(3):E413-9. (PMID: 11171595)
      J Biol Chem. 2006 Apr 28;281(17):11658-68. (PMID: 16517608)
      Trends Cell Biol. 2010 Feb;20(2):102-12. (PMID: 20006503)
      J Cell Sci. 2005 Dec 1;118(Pt 23):5411-9. (PMID: 16306220)
      Neuron. 2008 Dec 10;60(5):748-66. (PMID: 19081372)
      J Neurosci. 2007 Jul 11;27(28):7469-75. (PMID: 17626207)
      J Cereb Blood Flow Metab. 2001 Oct;21(10):1133-45. (PMID: 11598490)
      Curr Alzheimer Res. 2013 May 1;10(4):390-405. (PMID: 23545067)
      J Cell Biol. 1970 May;45(2):221-34. (PMID: 5513605)
      J Bioenerg Biomembr. 1999 Oct;31(5):419-30. (PMID: 10653471)
      J Neurosci Res. 2000 Jul 15;61(2):186-92. (PMID: 10878591)
      J Bioenerg Biomembr. 2013 Oct;45(5):431-40. (PMID: 23494666)
      Trends Mol Med. 2008 Feb;14(2):45-53. (PMID: 18218341)
      J Biol Chem. 1970 Sep 25;245(18):4724-31. (PMID: 4393961)
      Biochim Biophys Acta. 2010 Jan;1802(1):143-50. (PMID: 19747973)
      Free Radic Res. 2000 Mar;32(3):189-98. (PMID: 10730818)
      Neurochem Res. 1995 Dec;20(12):1465-70. (PMID: 8789609)
      Nat Rev Neurosci. 2008 Jul;9(7):505-18. (PMID: 18568013)
      Cell. 2004 Dec 17;119(6):873-87. (PMID: 15607982)
      J Biol Chem. 1951 Nov;193(1):265-75. (PMID: 14907713)
      Life Sci. 1977 Sep 1;21(5):637-45. (PMID: 904440)
      Neuroscience. 2011 Aug 11;188:117-24. (PMID: 21600964)
      Eur J Biochem. 1994 Mar 15;220(3):871-9. (PMID: 8143741)
      Biochem Soc Trans. 2010 Apr;38(2):457-9. (PMID: 20298202)
      Brain Res. 2010 Mar 10;1319:44-53. (PMID: 20079718)
      Am J Physiol Regul Integr Comp Physiol. 2004 Mar;286(3):R505-11. (PMID: 14615275)
    • Contributed Indexing:
      Keywords: Aging; Cerebral cortex; Depolarization; Non-synaptic mitochondria; Respiration; Synaptosomes
    • Accession Number:
      SY7Q814VUP (Calcium)
    • Publication Date:
      Date Created: 20160129 Date Completed: 20161102 Latest Revision: 20181113
    • Publication Date:
      20221213
    • Accession Number:
      10.1007/s11064-015-1817-5
    • Accession Number:
      26818758