Diverse Array of New Viral Sequences Identified in Worldwide Populations of the Asian Citrus Psyllid (Diaphorina citri) Using Viral Metagenomics.

Publisher: American Society For Microbiology Country of Publication: United States NLM ID: 0113724 Publication Model: Electronic Cited Medium: Internet ISSN: 1098-5514 (Electronic) Linking ISSN: 0022538X NLM ISO Abbreviation: J Virol Subsets: MEDLINE

Publication: Washington Dc : American Society For Microbiology
Original Publication: Baltimore, American Society for Microbiology.

Biodiversity*; Hemiptera/*virology ; Insect Viruses/*classification ; Insect Viruses/*isolation & purification ; Metagenomics/*methods; Animals ; Cluster Analysis ; Computational Biology ; Gene Expression Profiling ; Insect Viruses/genetics ; Molecular Sequence Data ; Phylogeny ; Sequence Analysis, RNA

Unlabelled: The Asian citrus psyllid, Diaphorina citri, is the natural vector of the causal agent of Huanglongbing (HLB), or citrus greening disease. Together; HLB and D. citri represent a major threat to world citrus production. As there is no cure for HLB, insect vector management is considered one strategy to help control the disease, and D. citri viruses might be useful. In this study, we used a metagenomic approach to analyze viral sequences associated with the global population of D. citri. By sequencing small RNAs and the transcriptome coupled with bioinformatics analysis, we showed that the virus-like sequences of D. citri are diverse. We identified novel viral sequences belonging to the picornavirus superfamily, the Reoviridae, Parvoviridae, and Bunyaviridae families, and an unclassified positive-sense single-stranded RNA virus. Moreover, a Wolbachia prophage-related sequence was identified. This is the first comprehensive survey to assess the viral community from worldwide populations of an agricultural insect pest. Our results provide valuable information on new putative viruses, some of which may have the potential to be used as biocontrol agents.
Importance: Insects have the most species of all animals, and are hosts to, and vectors of, a great variety of known and unknown viruses. Some of these most likely have the potential to be important fundamental and/or practical resources. In this study, we used high-throughput next-generation sequencing (NGS) technology and bioinformatics analysis to identify putative viruses associated with Diaphorina citri, the Asian citrus psyllid. D. citri is the vector of the bacterium causing Huanglongbing (HLB), currently the most serious threat to citrus worldwide. Here, we report several novel viral sequences associated with D. citri.
(Copyright © 2016, American Society for Microbiology. All Rights Reserved.)

Virology. 2009 May 25;388(1):1-7. (PMID: 19394993)
Arch Virol. 2008;153(4):715-27. (PMID: 18293057)
mBio. 2011 Jun 14;2(3):e00077-11. (PMID: 21673192)
Curr Opin Microbiol. 2013 Aug;16(4):468-78. (PMID: 23725672)
J Gen Virol. 1992 Apr;73 ( Pt 4):785-9. (PMID: 1634874)
Viruses. 2011 Oct;3(10):1849-69. (PMID: 22069519)
PLoS One. 2011;6(9):e24758. (PMID: 21949749)
J Virol. 2012 Apr;86(8):4620-30. (PMID: 22345464)
J Virol. 2010 Dec;84(24):13004-18. (PMID: 20926577)
PLoS One. 2014 Mar 17;9(3):e92107. (PMID: 24637949)
PLoS One. 2011;6(6):e20579. (PMID: 21674005)
Protein Cell. 2010 Feb;1(2):109-14. (PMID: 21203979)
Curr Opin Insect Sci. 2015 Apr;8:54-61. (PMID: 32846679)
J Gen Virol. 2014 Dec;95(Pt 12):2809-2819. (PMID: 25170050)
Phytopathology. 2013 May;103(5):488-500. (PMID: 23268581)
Virology. 2011 May 25;414(1):42-50. (PMID: 21470650)
Nucleic Acids Res. 2006 Jan 18;34(2):462-71. (PMID: 16421273)
Mol Ecol. 2010 Mar;19 Suppl 1:81-8. (PMID: 20331772)
J Invertebr Pathol. 2010 Jan;103 Suppl 1:S120-31. (PMID: 19909978)
Virus Res. 2008 Mar;132(1-2):59-68. (PMID: 18079012)
Infect Genet Evol. 2014 Mar;22:134-41. (PMID: 24462907)
J Gen Virol. 2007 Sep;88(Pt 9):2590-2595. (PMID: 17698671)
PLoS One. 2012;7(5):e37127. (PMID: 22623984)
J Mol Evol. 2000 Nov;51(5):491-7. (PMID: 11080372)
J Virol. 2010 Jul;84(14):6955-65. (PMID: 20463061)
Proc Natl Acad Sci U S A. 2015 Jun 16;112(24):7536-41. (PMID: 26038576)
PLoS Pathog. 2008 Nov;4(11):e1000212. (PMID: 19023410)
PLoS One. 2014 Jan 29;9(1):e87194. (PMID: 24489870)
Mol Plant Pathol. 2009 Jul;10(4):537-45. (PMID: 19523106)
PLoS One. 2013 Nov 18;8(11):e80720. (PMID: 24260463)
FEMS Microbiol Lett. 2008 Apr;281(2):215-20. (PMID: 18312577)
Genome Announc. 2015 Apr 30;3(2):. (PMID: 25931595)
F1000 Biol Rep. 2012;4:17. (PMID: 22991582)
J Immunol. 2013 Jan 15;190(2):650-8. (PMID: 23255357)
J Virol. 2004 Sep;78(17):9243-56. (PMID: 15308719)
Curr Opin Virol. 2013 Apr;3(2):199-204. (PMID: 23602435)
Clin Microbiol Rev. 2006 Jul;19(3):531-45. (PMID: 16847084)
Virology. 1966 Apr;28(4):571-9. (PMID: 5938886)
J Gen Virol. 2014 Oct;95(Pt 10):2285-2296. (PMID: 24986084)
Virology. 2014 Jan 5;448:146-58. (PMID: 24314645)
Rev Med Virol. 2007 Mar-Apr;17(2):115-31. (PMID: 17295196)
Genome Announc. 2014 May 15;2(3):. (PMID: 24831145)
Arch Virol. 2014 Mar;159(3):585-8. (PMID: 24065216)
Annu Rev Entomol. 2013;58:413-32. (PMID: 23317046)
PLoS Pathog. 2014 Oct 30;10(10):e1004490. (PMID: 25357125)
Phytopathology. 2013 Oct;103(10):1077-86. (PMID: 23718835)
Nat Rev Microbiol. 2008 Dec;6(12):925-39. (PMID: 18997823)
Genome Res. 2008 May;18(5):821-9. (PMID: 18349386)
Plant Dis. 2004 Dec;88(12):1382. (PMID: 30795206)
PLoS One. 2014 Jan 21;9(1):e86748. (PMID: 24466220)
Virology. 2004 Mar 15;320(2):206-17. (PMID: 15016544)
Curr Opin Microbiol. 2013 Aug;16(4):507-13. (PMID: 23850098)
J Virol. 2013 Mar;87(5):2475-88. (PMID: 23255793)
Proc Natl Acad Sci U S A. 2010 Nov 9;107(45):19390-5. (PMID: 20978209)
Mol Biol Evol. 2013 Dec;30(12):2725-9. (PMID: 24132122)
J Virol Methods. 2010 Sep;168(1-2):223-7. (PMID: 20558208)
Sci Rep. 2014 May 30;4:5127. (PMID: 24875042)
J Gen Virol. 1991 Oct;72 ( Pt 10):2425-30. (PMID: 1919524)
Virology. 1991 Feb;180(2):518-26. (PMID: 1989382)
J Invertebr Pathol. 2012 Jan;109(1):11-9. (PMID: 21939663)
J Gen Virol. 2015 Aug;96(8):2442-2452. (PMID: 25900137)
Proc Natl Acad Sci U S A. 2012 Dec 18;109(51):E3604-13. (PMID: 23151511)
Virology. 1985 Jul 30;144(2):398-409. (PMID: 3840622)
J Gen Virol. 1998 Jun;79 ( Pt 6):1479-85. (PMID: 9634091)
Arch Virol. 2014 Feb;159(2):385-8. (PMID: 24005374)
Adv Virus Res. 2006;68:361-92. (PMID: 16997017)
Virology. 2013 Jul 20;442(1):12-9. (PMID: 23642540)
PLoS Pathog. 2013;9(8):e1003579. (PMID: 24009507)
Virus Res. 2011 Feb;155(2):397-405. (PMID: 21144872)
Contrib Microbiol. 2000;4:12-32. (PMID: 10941568)
Elife. 2015 Jan 29;4:. (PMID: 25633976)
Virus Res. 2013 Sep;176(1-2):179-87. (PMID: 23777870)
Nucleic Acids Res. 2015 Jul 27;43(13):6191-206. (PMID: 26040701)
Science. 2007 Oct 12;318(5848):283-7. (PMID: 17823314)
Virology. 2000 Nov 10;277(1):167-77. (PMID: 11062047)
J Invertebr Pathol. 2012 Jan;109(1):127-33. (PMID: 22041201)
J Virol. 2014 Aug;88(16):8783-94. (PMID: 24850747)
J Invertebr Pathol. 2003 Sep;84(1):6-14. (PMID: 13678707)
RNA. 2010 Aug;16(8):1540-58. (PMID: 20566670)
J Gen Virol. 2011 Nov;92(Pt 11):2467-2484. (PMID: 21865443)
N Engl J Med. 2008 Mar 6;358(10):991-8. (PMID: 18256387)
Virology. 2012 Oct 10;432(1):162-72. (PMID: 22749878)
Am J Trop Med Hyg. 2010 Sep;83(3):614-23. (PMID: 20810829)
Genome Announc. 2013 Nov 27;1(6):. (PMID: 24285660)
Mol Biol Evol. 2007 Feb;24(2):427-35. (PMID: 17095536)
Proc Natl Acad Sci U S A. 2010 Jan 26;107(4):1606-11. (PMID: 20080648)
Viruses. 2014 Jan 06;6(1):106-36. (PMID: 24399207)
PLoS Pathog. 2010 Feb 12;6(2):e1000764. (PMID: 20169186)
Insect Mol Biol. 2010 Feb;19 Suppl 1:99-107. (PMID: 20167021)
J Virol. 2006 May;80(10):5059-64. (PMID: 16641297)
PLoS One. 2013 Nov 18;8(11):e79777. (PMID: 24260298)
J Mol Biol. 1990 Oct 5;215(3):403-10. (PMID: 2231712)
PLoS Biol. 2015 Jul 14;13(7):e1002210. (PMID: 26172158)
Virus Res. 2012 Feb;163(2):503-11. (PMID: 22142476)

Date Created: 20151218 Date Completed: 20160620 Latest Revision: 20240325

20240325

PMC4810699

10.1128/JVI.02793-15

26676774