The proline transport protein of Aspergillus nidulans is very similar to amino acid transporters of Saccharomyces cerevisiae.

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  • Author(s): Sophianopoulou V;Sophianopoulou V; Scazzocchio C
  • Source:
    Molecular microbiology [Mol Microbiol] 1989 Jun; Vol. 3 (6), pp. 705-14.
  • Publication Type:
    Comparative Study; Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Blackwell Scientific Publications Country of Publication: England NLM ID: 8712028 Publication Model: Print Cited Medium: Print ISSN: 0950-382X (Print) Linking ISSN: 0950382X NLM ISO Abbreviation: Mol Microbiol Subsets: MEDLINE
    • Publication Information:
      Original Publication: Oxford, OX ; Boston, MA : Blackwell Scientific Publications, c1987-
    • Subject Terms:
      Amino Acid Transport Systems, Neutral*; Aspergillus nidulans/*genetics ; Carrier Proteins/*genetics ; Proline/*metabolism ; Saccharomyces cerevisiae/*genetics; Amino Acid Sequence ; Amino Acid Transport Systems ; Base Sequence ; Blotting, Northern ; Carrier Proteins/biosynthesis ; DNA, Fungal ; Genes, Fungal ; Membrane Proteins/genetics ; Molecular Sequence Data ; Multigene Family ; Restriction Mapping ; Transcription, Genetic
    • Abstract:
      In Aspergillus nidulans, the gene prnB encoding the major proline transport system is one of a cluster of four genes necessary and sufficient for the utilization of proline as sole nitrogen and/or carbon source. The prn cluster has been cloned and the sequence and transcript map of the prnB gene are presented in this paper. The predicted translated sequence consists of 570 amino acids, resulting in a molecular weight of 63,028 Daltons. Its hydropathy profile shows 10 hydrophobic segments typical of integral membrane proteins. No N-terminal hydrophobic signal peptide is present, the N-terminal and C-terminal ends of the protein being hydrophilic. Similar results were previously found for the arginine and histidine transporters of Saccharomyces cerevisiae, with which the prnB transporter shares regions of highly conserved amino acid sequences. Using S1 mapping and Northern blot analyses, we confirm the presence of a unique inducible prnB transcript of 1.9 kb.
    • Accession Number:
      0 (Amino Acid Transport Systems)
      0 (Amino Acid Transport Systems, Neutral)
      0 (Carrier Proteins)
      0 (DNA, Fungal)
      0 (Membrane Proteins)
      0 (proline transport protein)
      9DLQ4CIU6V (Proline)
    • Publication Date:
      Date Created: 19890601 Date Completed: 19890825 Latest Revision: 20190725
    • Publication Date:
      20240829
    • Accession Number:
      10.1111/j.1365-2958.1989.tb00219.x
    • Accession Number:
      2664423