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Analytical ultracentrifugation: A versatile tool for the characterisation of macromolecular complexes in solution.
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- Author(s): Patel TR;Patel TR; Winzor DJ; Winzor DJ; Scott DJ; Scott DJ; Scott DJ
- Source:
Methods (San Diego, Calif.) [Methods] 2016 Feb 15; Vol. 95, pp. 55-61. Date of Electronic Publication: 2015 Nov 10.
- Publication Type:
Journal Article; Research Support, Non-U.S. Gov't; Review
- Language:
English
- Additional Information
- Source:
Publisher: Academic Press Country of Publication: United States NLM ID: 9426302 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-9130 (Electronic) Linking ISSN: 10462023 NLM ISO Abbreviation: Methods Subsets: MEDLINE
- Publication Information:
Publication: Duluth, MN : Academic Press
Original Publication: San Diego : Academic Press, c1990-
- Subject Terms:
- Abstract:
Analytical ultracentrifugation, an early technique developed for characterizing quantitatively the solution properties of macromolecules, remains a powerful aid to structural biologists in their quest to understand the formation of biologically important protein complexes at the molecular level. Treatment of the basic tenets of the sedimentation velocity and sedimentation equilibrium variants of analytical ultracentrifugation is followed by considerations of the roles that it, in conjunction with other physicochemical procedures, has played in resolving problems encountered in the delineation of complex formation for three biological systems - the cytoplasmic dynein complex, mitogen-activated protein kinase (ERK2) self-interaction, and the terminal catalytic complex in selenocysteine synthesis.
(Copyright © 2015 Elsevier Inc. All rights reserved.)
- Grant Information:
BB/F011156/1 United Kingdom BB_ Biotechnology and Biological Sciences Research Council
- Contributed Indexing:
Keywords: Analytical ultracentrifugation; Catalytic complex in selenocysteine synthesis; Cytoplasmic dynein complex; Mitogen-activated protein kinase (ERK2); Sedimentation equilibrium; Sedimentation velocity; SepSecS–tRNA(Sec) interaction
- Accession Number:
0 (Macromolecular Substances)
0 (Solutions)
0CH9049VIS (Selenocysteine)
9014-25-9 (RNA, Transfer)
EC 2.7.11.24 (MAPK1 protein, human)
EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1)
EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
EC 3.6.4.2 (Cytoplasmic Dyneins)
EC 6.1.1.- (Amino Acyl-tRNA Synthetases)
EC 6.1.1.- (O-phosphoseryl-tRNA:selenocysteinyl-tRNA synthase, human)
- Publication Date:
Date Created: 20151112 Date Completed: 20161025 Latest Revision: 20220129
- Publication Date:
20231215
- Accession Number:
10.1016/j.ymeth.2015.11.006
- Accession Number:
26555086
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