Proteomics Analysis of Dorsal Striatum Reveals Changes in Synaptosomal Proteins following Methamphetamine Self-Administration in Rats.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read Online Read More Add to Saved list
  • Author(s): Bosch PJ;Bosch PJ; Peng L; Peng L; Kivell BM; Kivell BM
  • Source:
    PloS one [PLoS One] 2015 Oct 20; Vol. 10 (10), pp. e0139829. Date of Electronic Publication: 2015 Oct 20 (Print Publication: 2015).
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
    • Publication Information:
      Original Publication: San Francisco, CA : Public Library of Science
    • Subject Terms:
      Central Nervous System Stimulants/*administration & dosage ; Corpus Striatum/*metabolism ; Methamphetamine/*administration & dosage ; Synaptosomes/*metabolism; Animals ; Corpus Striatum/drug effects ; Male ; Proteomics ; Rats ; Rats, Sprague-Dawley ; Self Administration ; Synaptosomes/drug effects ; Tandem Mass Spectrometry
    • Abstract:
      Methamphetamine is a widely abused, highly addictive drug. Regulation of synaptic proteins within the brain's reward pathway modulates addiction behaviours, the progression of drug addiction and long-term changes in brain structure and function that result from drug use. Therefore, using large scale proteomics studies we aim to identify global protein expression changes within the dorsal striatum, a key brain region involved in the modulation of addiction. We performed LC-MS/MS analyses on rat striatal synaptosomes following 30 days of methamphetamine self-administration (2 hours/day) and 14 days abstinence. We identified a total of 84 differentially-expressed proteins with known roles in neuroprotection, neuroplasticity, cell cytoskeleton, energy regulation and synaptic vesicles. We identify significant expression changes in stress-induced phosphoprotein and tubulin polymerisation-promoting protein, which have not previously been associated with addiction. In addition, we confirm the role of amphiphysin and phosphatidylethanolamine binding protein in addiction. This approach has provided new insight into the effects of methamphetamine self-administration on synaptic protein expression in a key brain region associated with addiction, showing a large set of differentially-expressed proteins that persist into abstinence. The mass spectrometry proteomics data are available via ProteomeXchange with identifier PXD001443.
    • References:
      Addict Biol. 2008 Sep;13(3-4):287-94. (PMID: 18279499)
      Proc Natl Acad Sci U S A. 2010 Jul 20;107(29):13147-52. (PMID: 20615969)
      Psychopharmacology (Berl). 2004 Aug;175(1):68-75. (PMID: 15029471)
      Anal Chem. 2002 Oct 15;74(20):5383-92. (PMID: 12403597)
      J Neurosci. 2001 Dec 1;21(23):9414-8. (PMID: 11717374)
      Brain Res. 2010 Mar 4;1317:137-46. (PMID: 19962371)
      Microbiol Mol Biol Rev. 2006 Mar;70(1):37-120. (PMID: 16524918)
      Pharmacogenomics J. 2005;5(3):203-14. (PMID: 15852055)
      Proteomics. 2005 Jul;5(10):2531-41. (PMID: 15984043)
      Neuron. 2002 Feb 28;33(5):789-804. (PMID: 11879655)
      Proteomics. 2010 Sep;10(18):3272-91. (PMID: 20718007)
      Proteomics. 2011 Nov;11(22):4376-84. (PMID: 21887821)
      Nat Neurosci. 2005 Nov;8(11):1481-9. (PMID: 16251991)
      Nat Protoc. 2008;3(11):1718-28. (PMID: 18927557)
      J Exp Biol. 2006 Feb;209(Pt 4):577-89. (PMID: 16449553)
      Free Radic Biol Med. 2012 Sep 1;53(5):1048-60. (PMID: 22796328)
      Brain Res. 2006 Jun 30;1097(1):19-25. (PMID: 16729985)
      Metab Brain Dis. 2009 Dec;24(4):685-700. (PMID: 19826936)
      Methods Mol Biol. 1997;72:33-47. (PMID: 9249736)
      Methods Mol Biol. 2010;662:303-16. (PMID: 20824478)
      J Neuropsychiatry Clin Neurosci. 2003 Summer;15(3):317-25. (PMID: 12928507)
      PLoS One. 2010;5(1):e8790. (PMID: 20098750)
      J Neural Transm (Vienna). 2013 Jul;120(7):1039-52. (PMID: 23224692)
      Brain Res Rev. 2009 May;60(2):379-407. (PMID: 19328213)
      Neuropharmacology. 2003 Apr;44(5):686-97. (PMID: 12668054)
      Drug Alcohol Depend. 2015 Jul 1;152:15-23. (PMID: 25962787)
      J Biol Chem. 2001 Jan 5;276(1):535-40. (PMID: 11034991)
      Nat Biotechnol. 2014 Mar;32(3):223-6. (PMID: 24727771)
      Pharmacol Biochem Behav. 1998 Sep;61(1):35-44. (PMID: 9715805)
      Mol Neurobiol. 2011 Dec;44(3):269-86. (PMID: 21922273)
      FEBS Lett. 2013 Jan 16;587(2):238-44. (PMID: 23238082)
      Anal Chem. 2003 Sep 1;75(17):4646-58. (PMID: 14632076)
      J Neurosci. 2013 Nov 6;33(45):17641-6. (PMID: 24198356)
      Proteomics. 2007 Apr;7(7):1131-9. (PMID: 17351886)
      J Cell Biol. 1994 Dec;127(6 Pt 2):1871-83. (PMID: 7806566)
      Neurochem Int. 2008 Jan;52(1-2):256-64. (PMID: 17904249)
      J Neurosci Res. 2012 Apr;90(4):895-904. (PMID: 22420045)
      Eur J Neurosci. 1999 May;11(5):1598-604. (PMID: 10215912)
      J Pharmacol Exp Ther. 2009 Nov;331(2):555-62. (PMID: 19648469)
      J Proteome Res. 2006 Nov;5(11):2909-18. (PMID: 17081042)
      Subcell Biochem. 2007;43:77-98. (PMID: 17953392)
      Cell. 2010 Oct 29;143(3):430-41. (PMID: 21029864)
      Proteomics Clin Appl. 2009 Mar 17;3(4):462-472. (PMID: 20161123)
    • Accession Number:
      0 (Central Nervous System Stimulants)
      44RAL3456C (Methamphetamine)
    • Publication Date:
      Date Created: 20151021 Date Completed: 20160607 Latest Revision: 20181113
    • Publication Date:
      20231215
    • Accession Number:
      PMC4618287
    • Accession Number:
      10.1371/journal.pone.0139829
    • Accession Number:
      26484527