Optimization of diacylglycerol production by glycerolysis of fish oil catalyzed by Lipozyme TL IM with Tween 65.

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  • Additional Information
    • Source:
      Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 101088505 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1615-7605 (Electronic) Linking ISSN: 16157591 NLM ISO Abbreviation: Bioprocess Biosyst Eng Subsets: MEDLINE
    • Publication Information:
      Original Publication: Berlin, Germany : Springer-Verlag, 2001-
    • Subject Terms:
      Diglycerides/*biosynthesis ; Fish Oils/*metabolism ; Glycerol/*metabolism ; Lipase/*metabolism ; Surface-Active Agents/*chemistry; Catalysis ; Hydrolysis
    • Abstract:
      The diacylglycerols (DAG) are emulsifiers with high added value used as functional additives in food, medicine, and cosmetic industries. In glycerolysis of oils for the production of DAG, the immiscibility between the substrates (glycerol and oil phases) has to be overcome, for example, by the addition of a food grade surfactant like Tween 65. The main objective of this work was to optimize the process conditions for obtaining diacylglycerols rich in the omega-3 eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids, through the enzymatic glycerolysis of fish oil, in systems with Tween 65 and without this surfactant, using Lipozyme TL(®) IM as biocatalyst. The experiments were performed according to predetermined conditions varying the temperature, enzyme load, the oil to glycerol molar ratio and, when added, the surfactant load. After 6 h of reaction, it was possible to produce up to 20.76 and 13.76% of diacylglycerols from fish oil in the reactions with and without Tween 65, respectively.
    • Contributed Indexing:
      Keywords: Biocatalysis; Diacylglycerols; Lipase; Omega-3; Surfactant
    • Accession Number:
      0 (Diglycerides)
      0 (Fish Oils)
      0 (Surface-Active Agents)
      EC 3.1.1.- (Lipozyme)
      EC 3.1.1.3 (Lipase)
      PDC6A3C0OX (Glycerol)
    • Publication Date:
      Date Created: 20150923 Date Completed: 20160902 Latest Revision: 20151029
    • Publication Date:
      20231215
    • Accession Number:
      10.1007/s00449-015-1473-9
    • Accession Number:
      26391508