[PECULIARITIES OF GLUCOSE AND GLYCEROL METABOLISM IN Nocardia vaccinii IMB B-7405].

Publisher: Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine Country of Publication: Ukraine NLM ID: 101657176 Publication Model: Print Cited Medium: Print ISSN: 2409-4943 (Print) Linking ISSN: 24094943 NLM ISO Abbreviation: Ukr Biochem J Subsets: MEDLINE

Original Publication: Kyiv : Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, 2014-

Bacterial Proteins/*metabolism ; Glucose/*metabolism ; Glycerol/*metabolism ; Nocardia/*metabolism; Citric Acid Cycle/physiology ; Dihydroxyacetone/metabolism ; Dihydroxyacetone Phosphate/metabolism ; Gluconates/metabolism ; Gluconeogenesis/physiology ; Glucose 1-Dehydrogenase/metabolism ; Glucosephosphate Dehydrogenase/metabolism ; Glutamate Dehydrogenase (NADP+)/metabolism ; Glycerophosphates/metabolism ; Glycolysis/physiology ; Ketoglutaric Acids/metabolism ; Ketone Oxidoreductases/metabolism ; Pentose Phosphate Pathway/physiology ; Phosphoenolpyruvate/metabolism ; Phosphoenolpyruvate Carboxykinase (ATP)/metabolism ; Phosphoenolpyruvate Carboxylase/metabolism ; Phosphogluconate Dehydrogenase/metabolism ; Phosphotransferases (Alcohol Group Acceptor)/metabolism ; Phosphotransferases (Paired Acceptors)/metabolism

It has been established that in cells of Nocardia vaccinii IMB B-7405 (surfactant producer) glucose catabolism is performed through pentose phosphate cycle as well as through gluconate (activity of NAD+-dependent glucose-6-phosphate dehydrogenase and FAD+-dependent glucose dehydrogenase 835 ± 41 and 698 ± 35 nmol.min-1.mg-1 of protein respectively). 6-Phosphogluconate formed in the gluconokinase reaction is involved in the pentose phosphate cycle (activity of constitutive NADP+-dependent 6-phosphogluconate dehydrogenase 357 ± 17 nmol.min-1.mg-1 of protein). Glycerol catabolism to dihydroxyacetonephosphate (the intermediate of glycolysis) may be performed in two ways: through glycerol-3-phosphate (glycerol kinase activity 244 ± 12 nmol.min-1.mg-1 of protein) and through dihydroxyacetone. Replenishment of the C4-dicarboxylic acids pool in N. vaccinii IMV B-7405 grown on glucose and glycerol occurs in the phosphoenolpyruvate(PEP)carboxylase reaction (714-803 nmol.min-1.mg-1 of protein). 2-Oxoglutarate was involved in tricarboxylic acid cycle by alternate pathway with the participation of 2-oxoglutarate synthase. The observed activity of both key enzymes of gluconeogenesis (PEP-carboxykinase and PEP-synthase), trehalose phosphate synthase and NADP+-dependent glutamate dehydrogenase confirmed the ability of IMV B-7405 strain to the synthesis of surface active glycoand aminolipids, respectively.

0 (Bacterial Proteins)
0 (Gluconates)
0 (Glycerophosphates)
0 (Ketoglutaric Acids)
57-04-5 (Dihydroxyacetone Phosphate)
73-89-2 (Phosphoenolpyruvate)
9NTI6P3O4X (alpha-glycerophosphoric acid)
EC 1.1.1.43 (Phosphogluconate Dehydrogenase)
EC 1.1.1.47 (Glucose 1-Dehydrogenase)
EC 1.1.1.49 (Glucosephosphate Dehydrogenase)
EC 1.2.- (Ketone Oxidoreductases)
EC 1.2.7.3 (2-oxoglutarate synthase)
EC 1.4.1.4 (Glutamate Dehydrogenase (NADP+))
EC 2.7.1.- (Phosphotransferases (Alcohol Group Acceptor))
EC 2.7.1.12 (gluconokinase)
EC 2.7.9.- (Phosphotransferases (Paired Acceptors))
EC 2.7.9.2 (pyruvate, water dikinase)
EC 4.1.1.31 (Phosphoenolpyruvate Carboxylase)
EC 4.1.1.49 (Phosphoenolpyruvate Carboxykinase (ATP))
IY9XDZ35W2 (Glucose)
O10DDW6JOO (Dihydroxyacetone)
PDC6A3C0OX (Glycerol)
R4R8J0Q44B (gluconic acid)
W31WK7B8U0 (6-phosphogluconic acid)

Date Created: 20150811 Date Completed: 20150825 Latest Revision: 20181202

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