Split-inteins and their bioapplications.

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  • Author(s): Li Y;Li Y
  • Source:
    Biotechnology letters [Biotechnol Lett] 2015 Nov; Vol. 37 (11), pp. 2121-37. Date of Electronic Publication: 2015 Jul 08.
  • Publication Type:
    Journal Article; Review
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Kluwer Academic Publishers Country of Publication: Netherlands NLM ID: 8008051 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-6776 (Electronic) Linking ISSN: 01415492 NLM ISO Abbreviation: Biotechnol Lett Subsets: MEDLINE
    • Publication Information:
      Publication: 1999- : Dordrecht : Kluwer Academic Publishers
      Original Publication: [Kew, Eng., Science and Technology Letters]
    • Subject Terms:
      Protein Splicing* ; Recombinant Proteins*/chemistry ; Recombinant Proteins*/isolation & purification ; Recombinant Proteins*/metabolism; Inteins/*genetics ; Protein Engineering/*methods; Biotechnology/methods
    • Abstract:
      Split-inteins are a subset of inteins that are expressed in two separate halves and catalyze splicing in trans upon association of the two domains. They occur naturally and have also been artificially generated by splitting of contiguous ones. With their unique properties, split-inteins offer improved controllability, flexibility and capability to existing tools based on contiguous inteins. In addition, split-inteins have proven useful in several new applications. This review gives a general introduction to split-inteins with a focus on their role in expanding the applications of intein-based technologies.
    • Contributed Indexing:
      Keywords: Expressed protein ligation (EPL); Protein purification; Protein trans-splicing (PTS); Site-specific labeling; Split-intein; Trans-cleavage
    • Accession Number:
      0 (Recombinant Proteins)
    • Publication Date:
      Date Created: 20150709 Date Completed: 20160623 Latest Revision: 20150925
    • Publication Date:
      20221213
    • Accession Number:
      10.1007/s10529-015-1905-2
    • Accession Number:
      26153348