A new colorimetric platform for ultrasensitive detection of protein and cancer cells based on the assembly of nucleic acids and proteins.

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  • Additional Information
    • Source:
      Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0370534 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-4324 (Electronic) Linking ISSN: 00032670 NLM ISO Abbreviation: Anal Chim Acta Subsets: MEDLINE
    • Publication Information:
      Publication: Amsterdam : Elsevier
      Original Publication: Amsterdam.
    • Subject Terms:
      Spectrophotometry, Ultraviolet*; Biotin/*chemistry ; DNA, Neoplasm/*analysis ; alpha-Fetoproteins/*analysis; Antibodies, Monoclonal/chemistry ; Antibodies, Monoclonal/immunology ; Biosensing Techniques ; Biotin/immunology ; Biotin/metabolism ; Biotinylation ; DNA, Neoplasm/chemistry ; HEK293 Cells ; HeLa Cells ; Horseradish Peroxidase/metabolism ; Humans ; Hydrogen Peroxide/metabolism ; Streptavidin/chemistry ; Streptavidin/metabolism ; alpha-Fetoproteins/chemistry
    • Abstract:
      An amplified colorimetric method has been developed for the detection of protein and cancer cells based on the assembly of nucleic acids and proteins for the first time. In this process, the assembly of nucleic acids was triggered by a biotinylated DNA strand after a sandwich immunoreaction. The biotinylated DNA strand and sandwich immunocomplex were connected by streptavidin. Then, the assembly of biotinylated bovine serum albumin (Biotin-BSA) and streptavidin-horseradish peroxidase (SA-HRP) occurred at a node of the assembled products of nucleic acids through the biotin-streptavidin reaction. Under the catalysis of horseradish peroxidase, 3,3',5,5'-tetramethylbenzidine (TMB) was oxidized by H2O2 and the oxidized product was analyzed by its UV-vis absorbance signal and sensitive colorimetric detection. This colorimetric sensor could not only achieve the quantitative determination of protein by UV-vis absorbance but could also be applied for semiquantitative determination by digital visualization. Using alpha-fetoprotein (AFP) as the model target, this proposed colorimetric method showed a wide linear range from 5 pg/mL to 1 ng/mL with a detection limit of 1.95 pg/mL by the instrument, and even 5 pg/mL target protein could be distinguished simply by the naked eye. This approach was then expanded to detect cancer cells based on the recognition of folic acid receptors that were over-expressed on the cancer cells by folic acid-tethered DNA. More importantly, this strategy can be further used as a universal colorimetric method for the determination of viruses or other proteins by changing the corresponding antibodies.
      (Copyright © 2015 Elsevier B.V. All rights reserved.)
    • Contributed Indexing:
      Keywords: Amplification; Assembly; Colorimetric; Protein detection
    • Accession Number:
      0 (Antibodies, Monoclonal)
      0 (DNA, Neoplasm)
      0 (alpha-Fetoproteins)
      6SO6U10H04 (Biotin)
      9013-20-1 (Streptavidin)
      BBX060AN9V (Hydrogen Peroxide)
      EC 1.11.1.- (Horseradish Peroxidase)
    • Publication Date:
      Date Created: 20150621 Date Completed: 20160323 Latest Revision: 20150620
    • Publication Date:
      20240829
    • Accession Number:
      10.1016/j.aca.2015.05.010
    • Accession Number:
      26092332