Identification and Qualification of Glycine Betaine, Proline and Trigonelline in Grape Leaves by HPLC.

The concentration of glycine betaine, proline and trigonelline in grape leaves is close relative to the ability of grapes' anti-stress. They were extracted by ion-exchange resin and tested by HPLC. This paper developed the method of HPLC, which included column temperature: 85 °C ,run time: 70 min, inject volume:100 µL, flow rate:0.6 mL/min, λ: 195 nm, column: suger-pak 1 (waters), mobile phase :50 mg/L Ca-EDTA. The method is stable. The relationship coefficients of glycine betaine, proline and trigonelline were respectively 0.9999,0.9994 and 0.9994, the retention time were 20.28,25.66 and 30.65 min, and detection limits were 1.35,2.56 and 1.02 ng/mL by the method of HPLC. Recoveries were 93% ∼ 96%. [ABSTRACT FROM AUTHOR]

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