[Effect of irradiated human lung fibroblasts on activation of canonical Wnt/β-catenin signaling pathway in mesenchymal stem cells].

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  • Additional Information
    • Source:
      Publisher: Zhejiang da xue xue bao bian ji bu Country of Publication: China NLM ID: 100927946 Publication Model: Print Cited Medium: Print ISSN: 1008-9292 (Print) Linking ISSN: 10089292 NLM ISO Abbreviation: Zhejiang Da Xue Xue Bao Yi Xue Ban Subsets: MEDLINE
    • Publication Information:
      Original Publication: Hangzhou : Zhejiang da xue xue bao bian ji bu, [1999]-
    • Subject Terms:
      Wnt Signaling Pathway*; Fibroblasts/*cytology ; Mesenchymal Stem Cells/*metabolism; Adaptor Proteins, Signal Transducing ; CCN Intercellular Signaling Proteins/metabolism ; Cells, Cultured ; Coculture Techniques ; Fibroblasts/radiation effects ; Gamma Rays ; Glycogen Synthase Kinase 3/metabolism ; Glycogen Synthase Kinase 3 beta ; Humans ; Intracellular Signaling Peptides and Proteins/metabolism ; Proto-Oncogene Proteins/metabolism ; Umbilical Cord/cytology ; X-Rays ; beta Catenin/metabolism
    • Abstract:
      Objective: To investigate the effect of irradiated human lung fibroblasts (HLFs) on the canonical Wnt/β-catenin signaling pathway in human umbilical cord mesenchymal stem cells (HUMSCs).
      Methods: HUMSCs were cultured alone (single group) or co-cultured with HLFs exposed to 5Gy X-rays (co-culture group). The protein levels of GSK-3β, p-GSK-3β, FRAT1 and β-catenin in HUMSCs were examined by Western blotting 3 days after culture or co-culture. WISP-1 protein levels in conditioned medium were examined by ELISA.
      Results: The levels of p-GSK3β/GSK3β (0.15 ± 0.05), FRAT1 (0.48 ± 0.07) and β-catenin (0.50 ± 0.07) in co-cultured HUMSCs significantly decreased compared to those in single group (0.55 ± 0.05, 1.16 ± 0.13 and 2.39 ± 0.15, all P<0.05). The supernatant level of WISP-1 in co-culture group was significantly decreased [(602.23 ± 161.47) ng/mL], compared to the single group [(977.77 ± 110.56) ng/mL, P<0.05].
      Conclusion: Irradiated HLFs attenuate the activation of canonical Wnt/β-catenin signaling pathway in HUMSCs in vitro.
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    • Accession Number:
      0 (Adaptor Proteins, Signal Transducing)
      0 (CCN Intercellular Signaling Proteins)
      0 (CCN4 protein, human)
      0 (CTNNB1 protein, human)
      0 (FRAT1 protein, human)
      0 (Intracellular Signaling Peptides and Proteins)
      0 (Proto-Oncogene Proteins)
      0 (beta Catenin)
      EC 2.7.11.1 (GSK3B protein, human)
      EC 2.7.11.1 (Glycogen Synthase Kinase 3 beta)
      EC 2.7.11.26 (Glycogen Synthase Kinase 3)
    • Publication Date:
      Date Created: 20150604 Date Completed: 20150726 Latest Revision: 20240922
    • Publication Date:
      20240922
    • Accession Number:
      PMC10396925
    • Accession Number:
      10.3785/j.issn.1008-9292.2015.03.007
    • Accession Number:
      26038134