Triiodothyronine modulates the expression of leptin and adiponectin in 3T3-L1 adipocytes.

Publisher: Instituto de Ensino e Pesquisa Albert Einstein Country of Publication: Brazil NLM ID: 101281800 Publication Model: Print Cited Medium: Internet ISSN: 2317-6385 (Electronic) Linking ISSN: 16794508 NLM ISO Abbreviation: Einstein (Sao Paulo) Subsets: MEDLINE

Original Publication: São Paulo, SP : Instituto de Ensino e Pesquisa Albert Einstein

3T3-L1 Cells/*drug effects ; Adipocytes/*drug effects ; Adiponectin/*genetics ; Gene Expression/*drug effects ; Leptin/*genetics ; Triiodothyronine/*pharmacology; Adiponectin/analysis ; Analysis of Variance ; Animals ; Cell Differentiation/drug effects ; Cells, Cultured ; Leptin/analysis ; Mice ; Obesity/genetics ; RNA, Messenger/analysis ; RNA, Messenger/drug effects ; Real-Time Polymerase Chain Reaction ; Reference Values ; Time Factors ; Triiodothyronine/administration & dosage

Objective: To study the effect of different doses of triiodothyronine on gene expression of the adipokines leptin and adiponectin, at different times, and to evaluate the difference in expression between the two adipokines in each group.
Methods: 3T3-L1 adipocytes were incubated with triiodothyronine at physiological dose (10nM) and supraphysiological doses (100nM or 1,000nM), or without triiodothyronine (control, C) for 0.5, 6, or 24 hours. Leptin and adiponectin mRNA was detected using real-time polymerase chain reaction (RT-PCR). One-way analyses of variance, Tukey's test or Student's t test, were used to analyze data, and significance level was set at 5%.
Results: Leptin levels decreased in the 1,000nM-dose group after 0.5 hour. Adiponectin levels dropped in the 10nM-dose group, but increased at the 100nM dose. After 6 hours, both genes were suppressed in all hormone concentrations. After 24 hours, leptin levels increased at 10, 100 and 1,000nM groups as compared to the control group; and adiponectin levels increased only in the 100nM group as compared to the control group.
Conclusion: These results demonstrated fast actions of triiodothyronine on the leptin and adiponectin expression, starting at 0.5 hour, at a dose of 1,000nM for leptin and 100nM for adiponectin. Triiodothyronine stimulated or inhibited the expression of adipokines in adipocytes at different times and doses which may be useful to assist in the treatment of obesity, assuming that leptin is increased and adiponectin is decreased, in obesity cases.

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0 (Adiponectin)
0 (Leptin)
0 (RNA, Messenger)
06LU7C9H1V (Triiodothyronine)

Date Created: 20150521 Date Completed: 20150929 Latest Revision: 20191210

20240829

PMC4946815

10.1590/S1679-45082015AO3068

25993072