Arabidopsis C3HC4-RING finger E3 ubiquitin ligase AtAIRP4 positively regulates stress-responsive abscisic acid signaling.

Publisher: Wiley-Blackwell Pub Country of Publication: China (Republic : 1949- ) NLM ID: 101250502 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1744-7909 (Electronic) Linking ISSN: 16729072 NLM ISO Abbreviation: J Integr Plant Biol Subsets: MEDLINE

Publication: [China] : Wiley-Blackwell Pub
Original Publication: [Carlton South, Victoria] : Blackwell Pub., 2005-

Signal Transduction*/drug effects ; Signal Transduction*/genetics ; Stress, Physiological*/drug effects ; Stress, Physiological*/genetics; Abscisic Acid/*metabolism ; Arabidopsis/*enzymology ; Arabidopsis/*physiology ; Arabidopsis Proteins/*metabolism ; Ubiquitin-Protein Ligases/*metabolism; Abscisic Acid/pharmacology ; Amino Acid Sequence ; Arabidopsis/drug effects ; Arabidopsis/genetics ; Arabidopsis Proteins/chemistry ; Arabidopsis Proteins/genetics ; Cytoplasm/enzymology ; Cytosol/metabolism ; DNA, Bacterial/genetics ; Droughts ; Gene Expression Regulation, Plant ; Genes, Plant ; Germination/drug effects ; Molecular Sequence Data ; Mutagenesis, Insertional/genetics ; Osmotic Pressure ; Phenotype ; Plant Roots/drug effects ; Plant Roots/growth & development ; Plant Stomata/drug effects ; Plant Stomata/physiology ; Seedlings/drug effects ; Seedlings/genetics ; Seedlings/growth & development ; Sodium Chloride/pharmacology ; Ubiquitin-Protein Ligases/chemistry ; Ubiquitin-Protein Ligases/genetics

Degradation of proteins via the ubiquitin system is an important step in many stress signaling pathways in plants. E3 ligases recognize ligand proteins and dictate the high specificity of protein degradation, and thus, play a pivotal role in ubiquitination. Here, we identified a gene, named Arabidopsis thaliana abscisic acid (ABA)-insensitive RING protein 4 (AtAIRP4), which is induced by ABA and other stress treatments. AtAIRP4 encodes a cellular protein with a C3HC4-RING finger domain in its C-terminal side, which has in vitro E3 ligase activity. Loss of AtAIRP4 leads to a decrease in sensitivity of root elongation and stomatal closure to ABA, whereas overexpression of this gene in the T-DNA insertion mutant atairp4 effectively recovered the ABA-associated phenotypes. AtAIRP4 overexpression plants were hypersensitive to salt and osmotic stresses during seed germination, and showed drought avoidance compared with the wild-type and atairp4 mutant plants. In addition, the expression levels of ABA- and drought-induced marker genes in AtAIRP4 overexpression plants were markedly higher than those in the wild-type and atairp4 mutant plants. Hence, these results indicate that AtAIRP4 may act as a positive regulator of ABA-mediated drought avoidance and a negative regulator of salt tolerance in Arabidopsis.
(© 2015 The Authors. Journal of Integrative Plant Biology published by Wiley Publishing Asia Pty Ltd on behalf of Institute of Botany, Chinese Academy of Sciences.)

Keywords: AtAIRP4; E3 ligase; abscisic acid; drought stress; salt stress

0 (AIRP4 protein, Arabidopsis)
0 (Arabidopsis Proteins)
0 (DNA, Bacterial)
0 (T-DNA)
451W47IQ8X (Sodium Chloride)
72S9A8J5GW (Abscisic Acid)
EC 2.3.2.27 (Ubiquitin-Protein Ligases)

Date Created: 20150428 Date Completed: 20161007 Latest Revision: 20220318

20240829

10.1111/jipb.12364

25913143