A Sensitive and Specific Indirect Competitive Enzyme-Linked Immunosorbent Assay for Detection of Paeoniflorin and Its Application in Pharmacokinetic Interactions between Paeoniflorin and Glycyrrhizinic Acid.

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  • Additional Information
    • Source:
      Publisher: George Thieme Country of Publication: Germany NLM ID: 0066751 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1439-0221 (Electronic) Linking ISSN: 00320943 NLM ISO Abbreviation: Planta Med Subsets: MEDLINE
    • Publication Information:
      Publication: Stuttgart ; New York : George Thieme
      Original Publication: Stuttgart, Hippokrates Verlag.
    • Subject Terms:
      Glucosides*/immunology ; Glucosides*/isolation & purification ; Glucosides*/pharmacokinetics ; Monoterpenes*/immunology ; Monoterpenes*/isolation & purification ; Monoterpenes*/pharmacokinetics; Enzyme-Linked Immunosorbent Assay/*methods ; Glycyrrhizic Acid/*pharmacokinetics; Animals ; Antibodies, Monoclonal ; Drug Interactions ; Female ; Hybridomas/immunology ; Mice ; Mice, Inbred BALB C
    • Abstract:
      This study aimed to develop an indirect competitive enzyme-linked immunosorbent assay based on monoclonal antibodies against paeoniflorin to study the effects of different doses of glycyrrhizinic acid on the pharmacokinetics of paeoniflorin in mice. An anti-paeoniflorin monoclonal antibody was produced from a hybridoma created through a fusion of splenocytes immunized with paeoniflorin-bovine serum albumin and conjugated with the hypoxanthine-aminopterin-thymidine-sensitive mouse myeloma cell line SP2/0. The resultant antibody was used to develop and validate a rapid, specific and sensitive, indirect competitive enzyme-linked immunosorbent assay for the measurement of paeoniflorin (linear range 4.8-312.5 ng · mL(-1)). The intraday and interday precision values of the indirect competitive ELISA method were well within the recommended range (≤ 10 %), and the recovery rate was, on average, 101.13 %. Pharmacokinetic parameters obtained from mouse blood samples at various intervals following the oral administration of paeoniflorin and glycyrrhizic acid at three doses (1 : 0.3, 1 : 1, 1 : 3, respectively) demonstrated that the highest dose of glycyrrhizic acid inhibits the absorption of paeoniflorin.
      (Georg Thieme Verlag KG Stuttgart · New York.)
    • Comments:
      Erratum in: Planta Med. 2015 Jun;81(9):E2. (PMID: 26110338)
    • Accession Number:
      0 (Antibodies, Monoclonal)
      0 (Glucosides)
      0 (Monoterpenes)
      21AIQ4EV64 (peoniflorin)
      6FO62043WK (Glycyrrhizic Acid)
    • Publication Date:
      Date Created: 20150410 Date Completed: 20160111 Latest Revision: 20150701
    • Publication Date:
      20231215
    • Accession Number:
      10.1055/s-0035-1545913
    • Accession Number:
      25856441