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Studying newt brain regeneration following subtype specific neuronal ablation.
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- Author(s): Kirkham M;Kirkham M; Joven A
- Source:
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2015; Vol. 1290, pp. 91-9.
- Publication Type:
Journal Article; Research Support, Non-U.S. Gov't
- Language:
English
- Additional Information
- Source:
Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE
- Publication Information:
Publication: Totowa, NJ : Humana Press
Original Publication: Clifton, N.J. : Humana Press,
- Subject Terms:
- Abstract:
The realization that neuronal injury does not result in permanent functional or cellular loss in all vertebrates has fascinated regenerative biologists. Neuronal regeneration occurs in a subset of species, including lizards, teleost fish, axolotls, and newts. One tool for studying neuronal regeneration in the adult brain is intraventricular injection of selective neuronal toxins, which leads to loss of subpopulations of neurons. To trace cells involved in the regeneration process, plasmids encoding reporter proteins can be electroporated in vivo into the cells of interest. This protocol describes methods to label the ependymoglial cells of the brain of the red spotted newt Notophthalmus viridescens and follow their response after ablation of dopaminergic neurons.
- Accession Number:
8HW4YBZ748 (Oxidopamine)
- Publication Date:
Date Created: 20150306 Date Completed: 20151125 Latest Revision: 20150305
- Publication Date:
20240829
- Accession Number:
10.1007/978-1-4939-2495-0_7
- Accession Number:
25740479
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