Blue-light dependent reactive oxygen species formation by Arabidopsis cryptochrome may define a novel evolutionarily conserved signaling mechanism.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Additional Information
    • Source:
      Publisher: Wiley on behalf of New Phytologist Trust Country of Publication: England NLM ID: 9882884 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1469-8137 (Electronic) Linking ISSN: 0028646X NLM ISO Abbreviation: New Phytol Subsets: MEDLINE
    • Publication Information:
      Publication: Oxford : Wiley on behalf of New Phytologist Trust
      Original Publication: London, New York [etc.] Academic Press.
    • Subject Terms:
      Biological Evolution* ; Light*; Arabidopsis/*metabolism ; Arabidopsis/*radiation effects ; Arabidopsis Proteins/*metabolism ; Cryptochromes/*metabolism ; Reactive Oxygen Species/*metabolism ; Signal Transduction/*radiation effects; Arabidopsis/drug effects ; Cell Death/drug effects ; Cell Survival/drug effects ; Cell Survival/radiation effects ; Oxygen/pharmacology ; Protoplasts/drug effects ; Protoplasts/metabolism ; Recombination, Genetic/genetics ; Sf9 Cells ; Signal Transduction/drug effects ; Subcellular Fractions/drug effects ; Subcellular Fractions/metabolism
    • Abstract:
      Cryptochromes are widespread blue-light absorbing flavoproteins with important signaling roles. In plants they mediate de-etiolation, developmental and stress responses resulting from interaction with downstream signaling partners such as transcription factors and components of the proteasome. Recently, it has been shown that Arabidopsis cry1 activation by blue light also results in direct enzymatic conversion of molecular oxygen (O2 ) to reactive oxygen species (ROS) and hydrogen peroxide (H2 O2 ) in vitro. Here we explored whether direct enzymatic synthesis of ROS by Arabidopsis cry1 can play a physiological role in vivo. ROS formation resulting from cry1 expression was measured by fluorescence assay in insect cell cultures and in Arabidopsis protoplasts from cryptochrome mutant seedlings. Cell death was determined by colorimetric assay. We found that ROS formation results from cry1 activation and induces cell death in insect cell cultures. In plant protoplasts, cryptochrome activation results in rapid increase in ROS formation and cell death. We conclude that ROS formation by cryptochromes may indeed be of physiological relevance and could represent a novel paradigm for cryptochrome signaling.
      (© 2015 The Authors. New Phytologist © 2015 New Phytologist Trust.)
    • Contributed Indexing:
      Keywords: Arabidopsis thaliana; cryptochrome; oxidative stress; photomorphogenesis; photoreceptor; reactive oxygen species (ROS) signaling
    • Accession Number:
      0 (Arabidopsis Proteins)
      0 (CRY1 protein, Arabidopsis)
      0 (Cryptochromes)
      0 (Reactive Oxygen Species)
      S88TT14065 (Oxygen)
    • Publication Date:
      Date Created: 20150303 Date Completed: 20160201 Latest Revision: 20200930
    • Publication Date:
      20231215
    • Accession Number:
      10.1111/nph.13341
    • Accession Number:
      25728686