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Isolation of mitochondria-associated ER membranes (MAMs) and glycosphingolipid-enriched microdomains (GEMs) from brain tissues and neuronal cells.
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- Author(s): Annunziata I;Annunziata I; Patterson A; d'Azzo A
- Source:
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2015; Vol. 1264, pp. 25-33.
- Publication Type:
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
- Language:
English
- Additional Information
- Source:
Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE
- Publication Information:
Publication: Totowa, NJ : Humana Press
Original Publication: Clifton, N.J. : Humana Press,
- Subject Terms:
- Abstract:
Subcellular fractionation is a valuable procedure in cell biology to separate and purify various subcellular constituents from one another, i.e., nucleus, cytosol, membranes/organelles, and cytoskeleton. The procedure relies on the use of differential centrifugation of cell and tissue homogenates, but additional purification steps now permit the isolation of inter-organellar membrane contact sites. Here, we outline a protocol tailored for the isolation of mitochondria, mitochondria-associated ER membranes (MAMs) and glycosphingolipid-enriched microdomains (GEMs) from the adult mouse brain, primary neurospheres, and murine embryonic fibroblasts (MEFs).
- Grant Information:
R01 DK052025 United States DK NIDDK NIH HHS; CA021764 United States CA NCI NIH HHS; DK52025 United States DK NIDDK NIH HHS; GM60905 United States GM NIGMS NIH HHS
- Accession Number:
0 (Glycosphingolipids)
- Publication Date:
Date Created: 20150130 Date Completed: 20151007 Latest Revision: 20170114
- Publication Date:
20231215
- Accession Number:
10.1007/978-1-4939-2257-4_3
- Accession Number:
25631000
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