Fascin1-dependent Filopodia are required for directional migration of a subset of neural crest cells.

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  • Additional Information
    • Source:
      Publisher: Public Library of Science Country of Publication: United States NLM ID: 101239074 Publication Model: eCollection Cited Medium: Internet ISSN: 1553-7404 (Electronic) Linking ISSN: 15537390 NLM ISO Abbreviation: PLoS Genet Subsets: MEDLINE
    • Publication Information:
      Original Publication: San Francisco, CA : Public Library of Science, c2005-
    • Subject Terms:
      Microfilament Proteins/*genetics ; Neural Crest/*growth & development ; Pseudopodia/*genetics ; Zebrafish/*growth & development ; Zebrafish Proteins/*genetics; Animals ; Body Patterning/genetics ; Cell Movement/genetics ; Epithelial-Mesenchymal Transition/genetics ; Gene Expression Regulation, Developmental ; Mutation ; Neural Crest/cytology ; Signal Transduction ; Zebrafish/genetics ; Zebrafish Proteins/biosynthesis
    • Abstract:
      Directional migration of neural crest (NC) cells is essential for patterning the vertebrate embryo, including the craniofacial skeleton. Extensive filopodial protrusions in NC cells are thought to sense chemo-attractive/repulsive signals that provide directionality. To test this hypothesis, we generated null mutations in zebrafish fascin1a (fscn1a), which encodes an actin-bundling protein required for filopodia formation. Homozygous fscn1a zygotic null mutants have normal NC filopodia due to unexpected stability of maternal Fscn1a protein throughout NC development and into juvenile stages. In contrast, maternal/zygotic fscn1a null mutant embryos (fscn1a MZ) have severe loss of NC filopodia. However, only a subset of NC streams display migration defects, associated with selective loss of craniofacial elements and peripheral neurons. We also show that fscn1a-dependent NC migration functions through cxcr4a/cxcl12b chemokine signaling to ensure the fidelity of directional cell migration. These data show that fscn1a-dependent filopodia are required in a subset of NC cells to promote cell migration and NC derivative formation, and that perdurance of long-lived maternal proteins can mask essential zygotic gene functions during NC development.
    • References:
      Mol Biol Cell. 2007 Nov;18(11):4591-602. (PMID: 17855511)
      Development. 1993 Dec;119(4):1203-15. (PMID: 8306883)
      Dev Cell. 2013 Feb 11;24(3):296-309. (PMID: 23375584)
      Development. 2009 Aug;136(15):2557-65. (PMID: 19592575)
      J Neurosci. 2010 Sep 29;30(39):13078-88. (PMID: 20881125)
      Cell. 2011 Jun 24;145(7):1012-22. (PMID: 21703446)
      Gene Expr Patterns. 2004 Oct;4(6):637-43. (PMID: 15465486)
      Cancer Cell. 2012 Mar 20;21(3):362-73. (PMID: 22439933)
      J Cell Sci. 2011 Oct 1;124(Pt 19):3305-18. (PMID: 21940796)
      Development. 2003 Dec;130(23):5755-68. (PMID: 14534133)
      Development. 2013 Aug;140(15):3198-209. (PMID: 23804498)
      Development. 2013 May;140(10):2203-11. (PMID: 23633513)
      Nucleic Acids Res. 2011 Jul;39(12):e82. (PMID: 21493687)
      Dev Biol. 1998 Nov 15;203(2):245-63. (PMID: 9808777)
      Commun Integr Biol. 2010 May;3(3):263-70. (PMID: 20714410)
      J Vis Exp. 2014;(84):e51138. (PMID: 24561516)
      J Neurosci. 2013 Jul 24;33(30):12171-85. (PMID: 23884926)
      J Cell Biol. 2006 Sep 11;174(6):863-75. (PMID: 16966425)
      Nat Neurosci. 2006 Dec;9(12):1506-11. (PMID: 17099706)
      Development. 2007 Feb;134(3):611-23. (PMID: 17215310)
      Development. 2014 Feb;141(4):929-39. (PMID: 24496629)
      Genome Res. 2011 Aug;21(8):1328-38. (PMID: 21555364)
      Dev Biol. 2010 Aug 15;344(2):543-54. (PMID: 20399765)
      Dev Biol. 2011 Dec 1;360(1):173-85. (PMID: 21963426)
      J Cell Sci. 1998 Jan;111 ( Pt 2):213-21. (PMID: 9405306)
      Nat Rev Mol Cell Biol. 2008 Jun;9(6):446-54. (PMID: 18464790)
      Cell Motil Cytoskeleton. 1998;40(2):119-32. (PMID: 9634210)
      PLoS One. 2013;8(5):e63074. (PMID: 23667577)
      Mech Dev. 2003 Feb;120(2):211-8. (PMID: 12559493)
      Development. 2009 Apr;136(8):1223-9. (PMID: 19304885)
      PLoS Genet. 2012;8(8):e1002861. (PMID: 22916025)
      Dev Cell. 2011 Dec 13;21(6):1026-37. (PMID: 22118769)
      Cell Adh Migr. 2010 Oct-Dec;4(4):586-94. (PMID: 20671421)
      Adv Exp Med Biol. 2006;589:213-34. (PMID: 17076285)
      Dev Biol. 1996 Dec 15;180(2):722-31. (PMID: 8954740)
      Eur J Cancer. 2013 Jan;49(1):219-30. (PMID: 22683307)
      Dev Biol. 2000 Sep 15;225(2):339-56. (PMID: 10985854)
      Biol Open. 2013 Sep 19;2(11):1187-91. (PMID: 24244855)
      Mol Biol Cell. 2014 Jul 15;25(14):2152-60. (PMID: 24829386)
      Curr Opin Cell Biol. 2001 Feb;13(1):76-84. (PMID: 11163137)
      Nat Rev Mol Cell Biol. 2009 Aug;10(8):538-49. (PMID: 19603038)
      Nucleic Acids Res. 2012 Jul;40(Web Server issue):W117-22. (PMID: 22693217)
      J Pathol. 2011 Jul;224(3):289-300. (PMID: 21618240)
      Wiley Interdiscip Rev Dev Biol. 2012 May-Jun;1(3):435-45. (PMID: 23801492)
      Sci STKE. 2007 Sep 8;2007(400):re5. (PMID: 17712139)
      J Cell Sci. 2013 Jan 1;126(Pt 1):312-26. (PMID: 23132923)
      Nature. 2008 Dec 18;456(7224):957-61. (PMID: 19078960)
      J Exp Zool B Mol Dev Evol. 2007 Sep 15;308(5):679-91. (PMID: 17724731)
      Development. 2009 Jun;136(12):1987-94. (PMID: 19439494)
      Dev Dyn. 2001 Feb;220(2):169-74. (PMID: 11169850)
      Development. 2013 Oct;140(19):4031-40. (PMID: 24046319)
      Nature. 2010 Apr 15;464(7291):1062-6. (PMID: 20393565)
      J Immunol. 2011 Mar 1;186(5):2850-9. (PMID: 21263068)
      Med Chem. 2011 May;7(3):155-64. (PMID: 21486209)
      J Biol Chem. 1997 Jan 24;272(4):2527-33. (PMID: 8999969)
      Dev Dyn. 2012 Jul;241(7):1155-68. (PMID: 22553120)
      Cell Motil Cytoskeleton. 2009 Aug;66(8):524-34. (PMID: 19343791)
      Dev Biol. 2006 Apr 1;292(1):174-88. (PMID: 16499899)
      Dev Biol. 2004 Feb 15;266(2):252-69. (PMID: 14738875)
      Dev Cell. 2013 Aug 12;26(3):292-302. (PMID: 23891662)
      Development. 2004 Dec;131(24):6141-51. (PMID: 15548586)
      Cell Adh Migr. 2011 Sep-Oct;5(5):421-30. (PMID: 21975551)
      PLoS One. 2013;8(10):e76789. (PMID: 24116159)
      Nat Rev Neurosci. 2003 Oct;4(10):806-18. (PMID: 14523380)
      Dis Model Mech. 2013 Jan;6(1):217-35. (PMID: 22917928)
      Dev Biol. 2009 Sep 1;333(1):161-72. (PMID: 19576198)
    • Grant Information:
      R01 DE13828 United States DE NIDCR NIH HHS; R01 DE013828 United States DE NIDCR NIH HHS; 5T32 HD07491 United States HD NICHD NIH HHS; T32 HD007491 United States HD NICHD NIH HHS; CDA 13-025 United States HX HSRD VA
    • Accession Number:
      0 (Microfilament Proteins)
      0 (Zebrafish Proteins)
      0 (fscn1a protein, zebrafish)
    • Publication Date:
      Date Created: 20150122 Date Completed: 20150630 Latest Revision: 20220331
    • Publication Date:
      20240829
    • Accession Number:
      PMC4301650
    • Accession Number:
      10.1371/journal.pgen.1004946
    • Accession Number:
      25607881