mir-233 modulates the unfolded protein response in C. elegans during Pseudomonas aeruginosa infection.

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  • Author(s): Dai LL;Dai LL; Gao JX; Gao JX; Zou CG; Zou CG; Ma YC; Ma YC; Zhang KQ; Zhang KQ
  • Source:
    PLoS pathogens [PLoS Pathog] 2015 Jan 08; Vol. 11 (1), pp. e1004606. Date of Electronic Publication: 2015 Jan 08 (Print Publication: 2015).
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Public Library of Science Country of Publication: United States NLM ID: 101238921 Publication Model: eCollection Cited Medium: Internet ISSN: 1553-7374 (Electronic) Linking ISSN: 15537366 NLM ISO Abbreviation: PLoS Pathog Subsets: MEDLINE
    • Publication Information:
      Original Publication: San Francisco, CA : Public Library of Science, c2005-
    • Subject Terms:
      Caenorhabditis elegans*/genetics ; Caenorhabditis elegans*/immunology ; Caenorhabditis elegans*/metabolism ; Pseudomonas Infections*/genetics ; Pseudomonas Infections*/immunology ; Pseudomonas Infections*/metabolism; MicroRNAs/*physiology ; Pseudomonas aeruginosa/*immunology ; Unfolded Protein Response/*genetics; Animals ; Animals, Genetically Modified ; Antigens, Ly/genetics ; Antigens, Ly/metabolism ; Gene Expression Profiling ; Gene Expression Regulation ; Immunity, Innate/genetics ; Microarray Analysis ; Pseudomonas aeruginosa/metabolism
    • Abstract:
      The unfolded protein response (UPR), which is activated by perturbations of the endoplasmic reticulum homeostasis, has been shown to play an important role in innate immunity and inflammation. However, little is known about the molecular mechanisms underlying activation of the UPR during immune responses. Using small RNA deep sequencing and reverse genetic analysis, we show that the microRNA mir-233 is required for activation of the UPR in Caenorhabditis elegans exposed to Pseudomonas aeruginosa PA14. P. aeruginosa infection up-regulates the expression of mir-233 in a p38 MAPK-dependent manner. Quantitative proteomic analysis identifies SCA-1, a C. elegans homologue of the sarco/endoplasmic reticulum Ca2+-ATPase, as a target of mir-233. During P. aeruginosa PA14 infection, mir-233 represses the protein levels of SCA-1, which in turn leads to activation of the UPR. Whereas mir-233 mutants are more sensitive to P. aeruginosa infection, knockdown of sca-1 leads to enhanced resistance to the killing by P. aeruginosa. Our study indicates that microRNA-dependent pathways may have an impact on innate immunity by activating the UPR.
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    • Accession Number:
      0 (Antigens, Ly)
      0 (MIRN233 microRNA, C elegans)
      0 (MicroRNAs)
    • Publication Date:
      Date Created: 20150109 Date Completed: 20151005 Latest Revision: 20210109
    • Publication Date:
      20221213
    • Accession Number:
      PMC4287614
    • Accession Number:
      10.1371/journal.ppat.1004606
    • Accession Number:
      25569229