Heat shock proteins: in vivo heat treatments reveal adipose tissue depot-specific effects.

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  • Additional Information
    • Source:
      Publisher: American Physiological Society Country of Publication: United States NLM ID: 8502536 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1522-1601 (Electronic) Linking ISSN: 01617567 NLM ISO Abbreviation: J Appl Physiol (1985) Subsets: MEDLINE
    • Publication Information:
      Original Publication: Bethesda, MD : American Physiological Society, c1985-
    • Subject Terms:
      Adipose Tissue, White/*metabolism ; Heat-Shock Proteins/*metabolism; Animals ; HSP72 Heat-Shock Proteins/metabolism ; Hot Temperature ; Lipolysis/physiology ; Male ; Organ Specificity ; Rats ; Rats, Wistar
    • Abstract:
      Heat treatments (HT) and the induction of heat shock proteins (HSPs) improve whole body and skeletal muscle insulin sensitivity while decreasing white adipose tissue (WAT) mass. However, HSPs in WAT have been understudied. The purpose of the present study was to examine patterns of HSP expression in WAT depots, and to examine the effects of a single in vivo HT on WAT metabolism. Male Wistar rats received HT (41°C, 20 min) or sham treatment (37°C), and 24 h later subcutaneous, epididymal, and retroperitoneal WAT depots (SCAT, eWAT, and rpWAT, respectively) were removed for ex vivo experiments and Western blotting. SCAT, eWAT, and rpWAT from a subset of rats were also cultured separately and received a single in vitro HT or sham treatment. HSP72 and HSP25 expression was greatest in more metabolically active WAT depots (i.e., eWAT and rpWAT) compared with the SCAT. Following HT, HSP72 increased in all depots with the greatest induction occurring in the SCAT. In addition, HSP25 increased in the rpWAT and eWAT, while HSP60 increased in the rpWAT only in vivo. Free fatty acid (FFA) release from WAT explants was increased following HT in the rpWAT only, and fatty acid reesterification was decreased in the rpWAT but increased in the SCAT following HT. HT increased insulin responsiveness in eWAT, but not in SCAT or rpWAT. Differences in HSP expression and induction patterns following HT further support the growing body of literature differentiating distinct WAT depots in health and disease.
      (Copyright © 2015 the American Physiological Society.)
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    • Grant Information:
      AG-031575 United States AG NIA NIH HHS; HD-002528 United States HD NICHD NIH HHS
    • Contributed Indexing:
      Keywords: HSP72; fatty acid reesterification; lipolysis; metabolism; stress response
    • Accession Number:
      0 (HSP72 Heat-Shock Proteins)
      0 (Heat-Shock Proteins)
    • Publication Date:
      Date Created: 20150103 Date Completed: 20150901 Latest Revision: 20220321
    • Publication Date:
      20221213
    • Accession Number:
      PMC4281645
    • Accession Number:
      10.1152/japplphysiol.00286.2014
    • Accession Number:
      25554799