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Macrophage migration inhibitory factor triggers chemotaxis of CD74+CXCR2+ NKT cells in chemically induced IFN-γ-mediated skin inflammation.
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- Additional Information
- Source:
Publisher: American Association of Immunologists Country of Publication: United States NLM ID: 2985117R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1550-6606 (Electronic) Linking ISSN: 00221767 NLM ISO Abbreviation: J Immunol Subsets: MEDLINE
- Publication Information:
Publication: Bethesda, MD : American Association of Immunologists
Original Publication: Baltimore : Williams & Wilkins, c1950-
- Subject Terms:
Antigens, Differentiation, B-Lymphocyte/
*immunology ;
Carcinogens/
*pharmacology ;
Drug Eruptions/
*immunology ;
Epidermis/
*immunology ;
Histocompatibility Antigens Class II/
*immunology ;
Interferon-gamma/
*immunology ;
Intramolecular Oxidoreductases/
*immunology ;
Keratinocytes/
*immunology ;
Macrophage Migration-Inhibitory Factors/
*immunology ;
Natural Killer T-Cells/
*immunology ;
Receptors, Interleukin-8B/
*immunology ;
Tetradecanoylphorbol Acetate/
*adverse effects;
Acute Disease ;
Animals ;
Antigens, Differentiation, B-Lymphocyte/
genetics ;
Cell Line ;
Chronic Disease ;
Disease Models, Animal ;
Drug Eruptions/
etiology ;
Drug Eruptions/
genetics ;
Drug Eruptions/
pathology ;
Epidermis/
pathology ;
Histocompatibility Antigens Class II/
genetics ;
Humans ;
Inflammation/
chemically induced ;
Inflammation/
genetics ;
Inflammation/
immunology ;
Inflammation/
pathology ;
Interferon-gamma/
genetics ;
Intramolecular Oxidoreductases/
genetics ;
Keratinocytes/
pathology ;
Macrophage Migration-Inhibitory Factors/
genetics ;
Mice ;
Natural Killer T-Cells/
pathology ;
Neovascularization, Pathologic/
chemically induced ;
Neovascularization, Pathologic/
genetics ;
Neovascularization, Pathologic/
immunology ;
Neovascularization, Pathologic/
pathology ;
Receptors, Interleukin-8B/
genetics ;
Tetradecanoylphorbol Acetate/
pharmacology - Abstract:
IFN-γ mediates chemically induced skin inflammation; however, the mechanism by which IFN-γ-producing cells are recruited to the sites of inflammation remains undefined. Secretion of macrophage migration inhibitory factor (MIF), a proinflammatory cytokine, from damaged cells may promote immune cell recruitment. We hypothesized that MIF triggers an initial step in the chemotaxis of IFN-γ-producing cells in chemically induced skin inflammation. Using acute and chronic models of 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced skin inflammation in mouse ears, MIF expression was examined, and its role in this process was investigated pharmacologically. The cell populations targeted by MIF, their receptor expression patterns, and the effects of MIF on cell migration were examined. TPA directly caused cytotoxicity accompanied by MIF release in mouse ear epidermal keratinocytes, as well as in human keratinocytic HaCaT cells. Treatment with the MIF antagonist (S,R)-3-(4-hydroxyphenyl)-4,5-dihydro-5-isoxazole acetic acid methyl ester considerably attenuated TPA-induced ear swelling, leukocyte infiltration, epidermal cell proliferation, and dermal angiogenesis. Inhibition of MIF greatly diminished the dermal infiltration of IFN-γ(+) NKT cells, whereas the addition of exogenous TPA and MIF to NKT cells promoted their IFN-γ production and migration, respectively. MIF specifically triggered the chemotaxis of NKT cells via CD74 and CXCR2, and the resulting depletion of NKT cells abolished TPA-induced skin inflammation. In TPA-induced skin inflammation, MIF is released from damaged keratinocytes and then triggers the chemotaxis of CD74(+)CXCR2(+) NKT cells for IFN-γ production.
(Copyright © 2014 by The American Association of Immunologists, Inc.)
- Accession Number:
0 (Antigens, Differentiation, B-Lymphocyte)
0 (Carcinogens)
0 (Histocompatibility Antigens Class II)
0 (IFNG protein, human)
0 (Macrophage Migration-Inhibitory Factors)
0 (Receptors, Interleukin-8B)
0 (invariant chain)
82115-62-6 (Interferon-gamma)
EC 5.3.- (Intramolecular Oxidoreductases)
EC 5.3.2.1 (MIF protein, human)
EC 5.3.2.1 (Mif protein, mouse)
NI40JAQ945 (Tetradecanoylphorbol Acetate)
- Publication Date:
Date Created: 20140831 Date Completed: 20141114 Latest Revision: 20140920
- Publication Date:
20221213
- Accession Number:
10.4049/jimmunol.1400692
- Accession Number:
25172501
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