The architecture of a scrambled genome reveals massive levels of genomic rearrangement during development.

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  • Additional Information
    • Source:
      Publisher: Cell Press Country of Publication: United States NLM ID: 0413066 Publication Model: Print Cited Medium: Internet ISSN: 1097-4172 (Electronic) Linking ISSN: 00928674 NLM ISO Abbreviation: Cell Subsets: MEDLINE
    • Publication Information:
      Publication: Cambridge, Ma : Cell Press
      Original Publication: Cambridge, MIT Press.
    • Subject Terms:
      Gene Rearrangement* ; Genome, Protozoan*; Oxytricha/*genetics ; Oxytricha/*growth & development; Cell Nucleus/metabolism ; Chromosomes/metabolism ; Molecular Sequence Data ; Oxytricha/cytology ; Oxytricha/metabolism
    • Abstract:
      Programmed DNA rearrangements in the single-celled eukaryote Oxytricha trifallax completely rewire its germline into a somatic nucleus during development. This elaborate, RNA-mediated pathway eliminates noncoding DNA sequences that interrupt gene loci and reorganizes the remaining fragments by inversions and permutations to produce functional genes. Here, we report the Oxytricha germline genome and compare it to the somatic genome to present a global view of its massive scale of genome rearrangements. The remarkably encrypted genome architecture contains >3,500 scrambled genes, as well as >800 predicted germline-limited genes expressed, and some posttranslationally modified, during genome rearrangements. Gene segments for different somatic loci often interweave with each other. Single gene segments can contribute to multiple, distinct somatic loci. Terminal precursor segments from neighboring somatic loci map extremely close to each other, often overlapping. This genome assembly provides a draft of a scrambled genome and a powerful model for studies of genome rearrangement.
      (Copyright © 2014 Elsevier Inc. All rights reserved.)
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    • Grant Information:
      GM59708 United States GM NIGMS NIH HHS; GM109459 United States GM NIGMS NIH HHS; R01 GM059708 United States GM NIGMS NIH HHS; R01 GM109459 United States GM NIGMS NIH HHS; T32 GM007388 United States GM NIGMS NIH HHS; F32 GM099462 United States GM NIGMS NIH HHS
    • Molecular Sequence:
      GENBANK ARYC00000000
    • Publication Date:
      Date Created: 20140830 Date Completed: 20150129 Latest Revision: 20211021
    • Publication Date:
      20221213
    • Accession Number:
      PMC4199391
    • Accession Number:
      10.1016/j.cell.2014.07.034
    • Accession Number:
      25171416