Ectopic expression of FaesAP3, a Fagopyrum esculentum (Polygonaceae) AP3 orthologous gene rescues stamen development in an Arabidopsis ap3 mutant.

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  • Author(s): Fang ZW;Fang ZW; Qi R; Qi R; Li XF; Li XF; Liu ZX; Liu ZX; Liu ZX
  • Source:
    Gene [Gene] 2014 Oct 25; Vol. 550 (2), pp. 200-6. Date of Electronic Publication: 2014 Aug 19.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Elsevier/North-Holland Country of Publication: Netherlands NLM ID: 7706761 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0038 (Electronic) Linking ISSN: 03781119 NLM ISO Abbreviation: Gene Subsets: MEDLINE
    • Publication Information:
      Original Publication: Amsterdam, Elsevier/North-Holland, 1976-
    • Subject Terms:
      Arabidopsis/*genetics ; Arabidopsis/*growth & development ; Arabidopsis Proteins/*genetics ; Fagopyrum/*genetics ; Flowers/*genetics ; Flowers/*growth & development ; MADS Domain Proteins/*genetics; Amino Acid Sequence ; Cloning, Molecular ; Flowers/metabolism ; Gene Expression Regulation, Developmental ; Gene Expression Regulation, Plant ; Gene Transfer Techniques ; Molecular Sequence Data ; Phylogeny ; Plants, Genetically Modified ; Sequence Homology
    • Abstract:
      Arabidopsis thaliana APETALA3 (AP3) and Antirrhinum majus DEFICIENS (DEF) MADS box genes are required to specify petal and stamen identity. AP3 and DEF are members of the euAP3 lineage, which arose by gene duplication coincident with radiation of the core eudicots. In order to investigate the molecular mechanisms underlying organ development in early diverging clades of core eudicots, we isolated and identified an AP3 homolog, FaesAP3, from Fagopyrum esculentum (buckwheat, Polygonaceae), a multi-food-use pseudocereal with healing benefits. Protein sequence alignment and phylogenetic analyses revealed that FaesAP3 grouped into the euAP3 lineage. Expression analysis showed that FaesAP3 was transcribed only in developing stamens, and differed from AP3 and DEF, which expressed in developing petals and stamens. Moreover, ectopic expression of FaesAP3 rescued stamen development without complementation of petal development in an Arabidopsis ap3 mutant. Our results suggest that FaesAP3 is involved in the development of stamens in buckwheat. These results also suggest that FaesAP3 holds some potential for biotechnical engineering to create a male sterile line of F. esculentum.
      (Copyright © 2014 Elsevier B.V. All rights reserved.)
    • Contributed Indexing:
      Keywords: APETALA3; B-function; Buckwheat; Floral development
    • Accession Number:
      0 (APETALA 3 protein, Arabidopsis)
      0 (Arabidopsis Proteins)
      0 (MADS Domain Proteins)
    • Publication Date:
      Date Created: 20140824 Date Completed: 20141121 Latest Revision: 20140908
    • Publication Date:
      20240829
    • Accession Number:
      10.1016/j.gene.2014.08.029
    • Accession Number:
      25149019