[Genome amplification and sequence analysis of human bocavirus 2].

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  • Author(s): Cui HD; Jin Y; Xie GC; Cheng WX; Duan ZJ
  • Source:
    Bing du xue bao = Chinese journal of virology [Bing Du Xue Bao] 2014 May; Vol. 30 (3), pp. 257-62.
  • Publication Type:
    English Abstract; Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    Chinese
  • Additional Information
    • Source:
      Publisher: Chung-Kuo Wei Sheng Wu Hsueh Hui Country of Publication: China NLM ID: 8803009 Publication Model: Print Cited Medium: Print ISSN: 1000-8721 (Print) Linking ISSN: 10008721 NLM ISO Abbreviation: Bing Du Xue Bao Subsets: MEDLINE
    • Publication Information:
      Publication: Beijing : Chung-Kuo Wei Sheng Wu Hsueh Hui
      Original Publication: [Peking] : Zhongguo wei sheng wu xue hui, 1985-
    • Subject Terms:
      Gene Amplification* ; Genome, Viral*; Human bocavirus/*genetics ; Parvoviridae Infections/*virology; Base Sequence ; Human bocavirus/chemistry ; Human bocavirus/classification ; Humans ; Molecular Sequence Data ; Nucleic Acid Conformation ; Phylogeny ; RNA, Viral/chemistry ; RNA, Viral/genetics ; Terminal Repeat Sequences
    • Abstract:
      To obtain the genome sequence of human bocavirus 2 (HBoV2), different regions of HBoV2 genome were amplified through PCR in fecal specimens which had been identified as single-positive for HBoV2 in 2010. A genome sequence of HBoV2 (HBoV2-NC, 5444 bp) was obtained after sequence assembly. The phylogenetic analysis showed that HBoV2-NC had the closest evolutionary relationship with HBoV2 Lanzhou strain. The predication of inverted terminal repeats of HBoV2-NC by DINAMelt showed that inverted terminal repeats were contained in HBoV2-NC 5' terminal, which had the typical stem-loop structure in other parvoviruses. Finally, some flanking sequences of HBoV2-NC were amplified by linker-PCR.
    • Accession Number:
      0 (RNA, Viral)
    • Publication Date:
      Date Created: 20140815 Date Completed: 20140902 Latest Revision: 20161021
    • Publication Date:
      20221213
    • Accession Number:
      25118380