The kinetic expression of lipopolysaccharide-binding protein and CD14 gene in obstructive jaundice.

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  • Author(s): Tsai CH;Tsai CH; Yeh CH; Sheen-Chen SM; Huang CY; Liu YW; Huang CC; Shen SC; Tang RP
  • Source:
    Journal of investigative surgery : the official journal of the Academy of Surgical Research [J Invest Surg] 2015 Feb; Vol. 28 (1), pp. 18-23. Date of Electronic Publication: 2014 Aug 05.
  • Publication Type:
    Comparative Study; Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Taylor & Francis Country of Publication: United States NLM ID: 8809255 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1521-0553 (Electronic) Linking ISSN: 08941939 NLM ISO Abbreviation: J Invest Surg Subsets: MEDLINE
    • Publication Information:
      Publication: [Philadelphia, PA] : Taylor & Francis
      Original Publication: New York : Taylor & Francis, c1988-
    • Subject Terms:
      Acute-Phase Proteins/*biosynthesis ; Carrier Proteins/*biosynthesis ; Jaundice, Obstructive/*metabolism ; Lipopolysaccharide Receptors/*biosynthesis ; Membrane Glycoproteins/*biosynthesis ; Tumor Necrosis Factor-alpha/*biosynthesis; Acute-Phase Proteins/genetics ; Animals ; Bile Ducts ; Carrier Proteins/genetics ; Ileum/metabolism ; Jaundice, Obstructive/genetics ; Ligation ; Lipopolysaccharide Receptors/genetics ; Liver/metabolism ; Lung/metabolism ; Male ; Membrane Glycoproteins/genetics ; RNA, Messenger/biosynthesis ; RNA, Messenger/genetics ; Rats ; Rats, Sprague-Dawley ; Spleen/metabolism ; Time Factors ; Tumor Necrosis Factor-alpha/genetics ; Up-Regulation
    • Abstract:
      Background: Binding lipopolysaccharide (LPS) with high-affinity, lipopolysaccharide-binding protein (LBP) and CD14 lower the threshold stimulatory concentrations of LPS dramatically and enhance the rate of cytokine production markedly. This study aimed to investigate the kinetic expression of LBP/CD14 and its possible relationship with tumor necrosis factor alpha (TNF-α) to better understand the pathophysiology of obstructive jaundice.
      Materials and Methods: The tissues (liver, spleen, intestine, and lung) of male Sprague-Dawley rats were harvested at pre-bile duct ligation in controls and at specific time points (24, 48, 72, 96, and 120 hr) after bile duct ligation. LBP, CD14, and TNF-α mRNA expression were measured in tissues harvested from controls and at the specific time points.
      Results: Hepatic LBP mRNA expression increased significantly at five days after bile duct ligation. CD 14 mRNA expression increased significantly after five days of bile duct ligation in liver, lung, spleen, and ileum. TNF-α mRNA expression increased significantly in all four organs (liver, lung, spleen, and ileum) after four days of bile duct ligation.
      Conclusion: Five days of bile duct ligation upregulated CD 14 mRNA expression in liver, lung, spleen, and ileum and increased TNF-α mRNA expression simultaneously in the liver, lung, spleen, and ileum. In addition, five days of bile duct ligation also upregulated LBP mRNA expression in the liver and increased hepatic TNF-α mRNA expression simultaneously. The kinetic expressions of LBP and CD 14 in obstructive jaundice are intriguing and further evaluation is warranted.
    • Contributed Indexing:
      Keywords: CD14; RNA; antigens; cytokines; genes; jaundice; lipopolysaccharide-binding protein; messenger; obstructive
    • Accession Number:
      0 (Acute-Phase Proteins)
      0 (Carrier Proteins)
      0 (Lipopolysaccharide Receptors)
      0 (Membrane Glycoproteins)
      0 (RNA, Messenger)
      0 (Tumor Necrosis Factor-alpha)
      0 (lipopolysaccharide-binding protein)
    • Publication Date:
      Date Created: 20140806 Date Completed: 20150907 Latest Revision: 20190116
    • Publication Date:
      20231215
    • Accession Number:
      10.3109/08941939.2014.943858
    • Accession Number:
      25093541