Ascorbic acid deficiency decreases hepatic cytochrome P-450, especially CYP2B1/2B2, and simultaneously induces heme oxygenase-1 gene expression in scurvy-prone ODS rats.

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  • Additional Information
    • Source:
      Publisher: Oxford University Press Country of Publication: England NLM ID: 9205717 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1347-6947 (Electronic) Linking ISSN: 09168451 NLM ISO Abbreviation: Biosci Biotechnol Biochem Subsets: MEDLINE
    • Publication Information:
      Publication: 2021- : Oxford : Oxford University Press
      Original Publication: Tokyo, Japan : Japan Society for Bioscience, Biotechnology, and Agrochemistry, c1992-
    • Subject Terms:
      Gene Expression Regulation, Enzymologic*; Aryl Hydrocarbon Hydroxylases/*metabolism ; Cytochrome P-450 CYP2B1/*metabolism ; Heme Oxygenase-1/*genetics ; Liver/*enzymology ; Scurvy/*enzymology ; Scurvy/*genetics ; Steroid Hydroxylases/*metabolism; Animals ; Disease Susceptibility ; Electron Transport Complex IV/metabolism ; Lipopolysaccharides/pharmacology ; Liver/drug effects ; Male ; Microsomes, Liver/drug effects ; Microsomes, Liver/enzymology ; Phenobarbital/pharmacology ; Rats ; Scurvy/metabolism
    • Abstract:
      The mechanisms underlying the decrease in hepatic cytochrome P-450 (CYP) content in ascorbic acid deficiency was investigated in scurvy-prone ODS rats. First, male ODS rats were fed a diet containing sufficient ascorbic acid (control) or a diet without ascorbic acid (deficient) for 18 days, with or without the intraperitoneal injection of phenobarbital. Ascorbic acid deficiency decreased hepatic microsomal total CYP content, CYP2B1/2B2 protein, and mitochondrial cytochrome oxidase (COX) complex IV subunit I protein, and simultaneously increased heme oxygenase-1 protein in microsomes and mitochondria. Next, heme oxygenase-1 inducers, that is lipopolysaccharide and hemin, were administered to phenobaribital-treated ODS rats fed sufficient ascorbic acid. The administration of these inducers decreased hepatic microsomal total CYP content, CYP2B1/2B2 protein, and mitochondrial COX complex IV subunit I protein. These results suggested that the stimulation of hepatic heme oxygenase-1 expression by ascorbic acid deficiency caused the decrease in CYP content in liver.
    • Contributed Indexing:
      Keywords: CYP2B1/2B2; ODS rat; ascorbic acid; cytochrome P-450; heme oxygenase-1
    • Accession Number:
      0 (Lipopolysaccharides)
      EC 1.14.- (Steroid Hydroxylases)
      EC 1.14.14.1 (Aryl Hydrocarbon Hydroxylases)
      EC 1.14.14.1 (Cytochrome P-450 CYP2B1)
      EC 1.14.14.1 (steroid 16-beta-hydroxylase)
      EC 1.14.14.18 (Heme Oxygenase-1)
      EC 1.9.3.1 (Electron Transport Complex IV)
      YQE403BP4D (Phenobarbital)
    • Publication Date:
      Date Created: 20140719 Date Completed: 20150330 Latest Revision: 20161125
    • Publication Date:
      20240829
    • Accession Number:
      10.1080/09168451.2014.910105
    • Accession Number:
      25036135