Anti-inflammatory activity and molecular mechanism of Oolong tea theasinensin.

Publisher: Royal Society of Chemistry Country of Publication: England NLM ID: 101549033 Publication Model: Print Cited Medium: Internet ISSN: 2042-650X (Electronic) Linking ISSN: 20426496 NLM ISO Abbreviation: Food Funct Subsets: MEDLINE

Original Publication: Cambridge : Royal Society of Chemistry

Anti-Inflammatory Agents/*pharmacology ; Benzopyrans/*pharmacology ; Phenols/*pharmacology ; Plant Preparations/*pharmacology ; Tea/*chemistry; Animals ; Cell Line, Tumor ; Chemokine CCL2/genetics ; Chemokine CCL2/metabolism ; Disease Models, Animal ; Down-Regulation ; Edema/chemically induced ; Edema/drug therapy ; Interleukin-12/metabolism ; Lipopolysaccharides/adverse effects ; MAP Kinase Kinase 1/genetics ; MAP Kinase Kinase 1/metabolism ; Macrophages/drug effects ; Macrophages/metabolism ; Male ; Mice ; Mice, Inbred ICR ; Nitric Oxide/metabolism ; Nitric Oxide Synthase Type II/genetics ; Nitric Oxide Synthase Type II/metabolism ; Tumor Necrosis Factor-alpha/metabolism

Oolong tea theasinensins are a group of tea polyphenols different from green tea catechins and black tea theaflavins, and they are considered as bioactive compounds in Oolong tea. In the present study, based on the properties of theasinensin and information about inflammatory processes, we investigated the anti-inflammatory activity and molecular mechanisms of theasinensin A (TSA) in both cell and animal models. In the cell model, TSA reduced the levels of pro-inflammatory mediators including inducible nitric oxide synthase (iNOS), nitric oxide (NO), interleukin-12 (IL-12) (p70), tumor necrosis factor alpha (TNF-α), and monocyte chemotactic protein-1 (MCP-1) induced by lipopolysaccharide (LPS). Cellular signaling analysis revealed that TSA downregulated MAPK/ERK kinase (MEK)-extracellular signal-regulated kinase (ERK) signaling. Pull-down assay and affinity data revealed that TSA might directly bind to MEK-ERK for the inhibitory action. In the animal model, TSA suppressed the production of IL-12 (p70), TNF-α, and MCP-1 and attenuated mouse paw edema induced by LPS.

0 (Anti-Inflammatory Agents)
0 (Benzopyrans)
0 (Ccl2 protein, mouse)
0 (Chemokine CCL2)
0 (Lipopolysaccharides)
0 (Phenols)
0 (Plant Preparations)
0 (Tea)
0 (Tumor Necrosis Factor-alpha)
0 (theasinensin A)
187348-17-0 (Interleukin-12)
31C4KY9ESH (Nitric Oxide)
EC 1.14.13.39 (Nitric Oxide Synthase Type II)
EC 1.14.13.39 (Nos2 protein, mouse)
EC 2.7.12.2 (MAP Kinase Kinase 1)
EC 2.7.12.2 (Map2k1 protein, mouse)

Date Created: 20140621 Date Completed: 20150330 Latest Revision: 20161125

20221213

10.1039/c4fo00152d

24947273