Production of novel "functional oil" rich in diglycerides and phytosterol esters with "one-pot" enzymatic transesterification.

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  • Additional Information
    • Source:
      Publisher: American Chemical Society Country of Publication: United States NLM ID: 0374755 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-5118 (Electronic) Linking ISSN: 00218561 NLM ISO Abbreviation: J Agric Food Chem Subsets: MEDLINE
    • Publication Information:
      Original Publication: Washington, American Chemical Society.
    • Subject Terms:
      Candida/*enzymology ; Diglycerides/*chemistry ; Esters/*chemistry ; Fungal Proteins/*chemistry ; Lipase/*chemistry ; Phytosterols/*chemistry ; Plant Oils/*chemistry; Enzyme Stability ; Enzymes, Immobilized/chemistry ; Esterification ; Functional Food/analysis ; Sunflower Oil ; Triglycerides/chemistry
    • Abstract:
      Diglycerides and phytosterol esters are two important functional lipids. Phytosterol esters mixed with dietary diglyceride could not only influence body weight but also prevent or reverse insulin resistance and hyperlipidemia. In this study, a kind of novel "functional oil" rich in both diglycerides and phytosterol esters was prepared with "one-pot" enzymatic transesterification. First, lipase AYS (Candida rugosa) was immobilized on the porous cross-linked polystyrene resin beads (NKA) via hydrophobic interaction. The resulting immobilized AYS showed much better transesterification activity and thermal stability to freeways. On the basis of the excellent biocatalyst prepared, a method for high-efficiency enzymatic esterification of phytosterols with different triglycerides to produce corresponding functional oils rich in both diglycerides and phytosterol esters was developed. Four functional oils rich in both diglycerides and phytosterol esters with conversions >92.1% and controllable fatty acid composition were obtained under the optimized conditions: 80 mmol/L phytosterols, 160 mmol/L triglycerides, and 25 mg/mL AYS@NKA at 180 rpm and 50 °C for 12 h in hexane. The prepared functional oil possessed low acid value (≤1.0 mgKOH/g), peroxide value (≤2.1 mmol/kg), and conjugated diene value (≤1.96 mmol/kg) and high diglyceride and phytosterol ester contents (≥10.4 and ≥20.2%, respectively). All of the characteristics favored the wide application of the functional oil in different fields of functional food.
    • Accession Number:
      0 (Diglycerides)
      0 (Enzymes, Immobilized)
      0 (Esters)
      0 (Fungal Proteins)
      0 (Phytosterols)
      0 (Plant Oils)
      0 (Sunflower Oil)
      0 (Triglycerides)
      EC 3.1.1.3 (Lipase)
    • Publication Date:
      Date Created: 20140513 Date Completed: 20150119 Latest Revision: 20171116
    • Publication Date:
      20221213
    • Accession Number:
      10.1021/jf500744n
    • Accession Number:
      24815553